TY - JOUR
T1 - The TFB4 subunit of yeast TFIIH is required for both nucleotide excision repair and RNA polymerase II transcription
AU - Feaver, William J.
AU - Huang, Wenya
AU - Friedberg, Errol C.
PY - 1999/10/8
Y1 - 1999/10/8
N2 - The N-degron strategy has been used to generate a yeast strain harboring a temperature-sensitive allele of TFB4 (tfb4(td)), the gene that encodes the 37-kDa subunit of the transcription/repair factor TFIIH. The tfb4(td) strain was sensitive to UV radiation and is defective in nucleotide excision repair in vitro. The mutant strain was also found to be an inositol auxotroph due at least in part to an inability to properly induce expression of the INO1 gene. These results indicate that like other subunits of TFIIH, Tfb4 is required for both RNA polymerase II transcription and DNA repair.
AB - The N-degron strategy has been used to generate a yeast strain harboring a temperature-sensitive allele of TFB4 (tfb4(td)), the gene that encodes the 37-kDa subunit of the transcription/repair factor TFIIH. The tfb4(td) strain was sensitive to UV radiation and is defective in nucleotide excision repair in vitro. The mutant strain was also found to be an inositol auxotroph due at least in part to an inability to properly induce expression of the INO1 gene. These results indicate that like other subunits of TFIIH, Tfb4 is required for both RNA polymerase II transcription and DNA repair.
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U2 - 10.1074/jbc.274.41.29564
DO - 10.1074/jbc.274.41.29564
M3 - Article
C2 - 10506223
AN - SCOPUS:0032855103
SN - 0021-9258
VL - 274
SP - 29564
EP - 29567
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 41
ER -