The thymidylate synthase gene of Hz-1 virus: A gene captured from its lepidopteran host

Hong Hwa Chen, Dan Ju Tso, Wen Bin Yeh, Hsien Jen Cheng, Ting Feng Wu

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


The sequence analysis of a thymidylate synthase gene was identified in the Hz-1 virus HindIII-D fragment. The viral thymidylate synthase gene encodes a protein of 295 amino acids, and is closely related to that of insect, mammals and herpesvirus. The thymidylate synthase gene identified was a genuine viral gene in that it was only detected in cells infected with Hz-1 virus but not in the mock infected cells, by Southern blot analysis and by RT-PCR. Results of phylogenetic analysis based on non-synonymous and amino acid distances suggested that the TS gene of Hz-1 virus was grouped closely with that of Bombyx mori. High bootstrap values confirmed that the thymidylate synthase of Hz-1 virus was acquired by a capture event from its lepidopteran host. Results of both sequence divergences and phylogenetic analysis suggested that TS genes in insect viruses, Hz-1, CIV, and MsEPV may have a different history or originated from different capture events.

Original languageEnglish
Pages (from-to)495-503
Number of pages9
JournalInsect Molecular Biology
Issue number5
Publication statusPublished - 2001

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Insect Science

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