The time window for therapy with peptide nanofibers combined with autologous bone marrow cells in pigs after acute myocardial infarction

Ming Yao Chang, Chih Han Chang, Chien Hsi Chen, Bill Cheng, Yi Dong Lin, Chwan Yau Luo, Hua Lin Wu, Yu Jen Yang, Jyh Hong Chen, Patrick C.H. Hsieh

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Background We previously showed that injection of peptide nanofibers (NF) combined with autologous bone marrow mononuclear cells (MNC) immediately after coronary artery ligation improves cardiac performance in pigs. To evaluate the clinical feasibility, this study was performed to determine the therapeutic time window for NF/MNC therapy in acute myocardial infarction (MI). Methods and Results A total of 45 adult minipigs were randomly grouped into 7 groups: sham or MI plus treatment with NS (normal saline), or NF or MNC alone at 1 day (1D) post-MI, or NF/MNC at 1, 4, or 7 days post-MI (N≥6). Cardiac function was assessed by echocardiography and ventricular catheterization. Compared with the NS control, pigs treated with NF/MNC at 1 day post-MI (NF/MC-1D) had the greatest improvement in left ventricle ejection fraction (LVEF; 55.1±1.6%; P<0.01 vs. NS) 2 months after MI. In contrast, pigs treated with either NF/MNC-4D or NF/ MNC-7D showed 48.9±0.8%(P<0.05 vs. NS) and 43.5±2.3%(n.s. vs. NS) improvements, respectively. The +dP/dt and -dP/dt, infarct size and interstitial collagen content were also improved in the NF/MNC-1D and -4D groups but not in the -7D group. Mechanistically,MNC quality and the states of systemic inflammation and damaged heart tissue influence the therapeutic efficiency of NF/MNC therapy, as revealed by another independent study using 16 pigs. PLOS ONE Conclusions Injection of NF/MNC at 1 or 4 days, but not at 7 days post-MI, improves cardiac performance and prevents ventricular remodeling, confirming the importance of early intervention when using this therapy for acute MI.

Original languageEnglish
Article numbere0115430
JournalPloS one
Volume10
Issue number3
DOIs
Publication statusPublished - 2015 Mar 1

All Science Journal Classification (ASJC) codes

  • General

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