The transcriptional terminator sequences downstream of the covR gene terminate covR/S operon transcription to generate covR monocistronic transcripts in Streptococcus pyogenes

Chuan Chiang-Ni, Chih Cheng Tsou, Yee-Shin Lin, Woei-Jer Chuang, Ming T. Lin, Ching-Chuan Liu, Jiunn Jong Wu

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

CovR/S is an important two component regulatory system, which regulates about 15% of the gene expression in Streptococcus pyogenes. The covR/S locus was identified as an operon generating an RNA transcript around 2.5-kb in size. In this study, we found the covR/S operon produced three RNA transcripts (around 2.5-, 1.0-, and 0.8-kb in size). Using RNA transcriptional terminator sequence prediction and transcriptional terminator analysis, we identified two atypical rho-independent terminator sequences downstream of the covR gene and showed these terminator sequences terminate RNA transcription efficiently. These results indicate that covR/S operon generates covR/S transcript and monocistronic covR transcripts.

Original languageEnglish
Pages (from-to)99-103
Number of pages5
JournalGene
Volume427
Issue number1-2
DOIs
Publication statusPublished - 2008 Dec 31

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Genetic Terminator Regions
Streptococcus pyogenes
Operon
RNA
Genes
Gene Expression

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

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title = "The transcriptional terminator sequences downstream of the covR gene terminate covR/S operon transcription to generate covR monocistronic transcripts in Streptococcus pyogenes",
abstract = "CovR/S is an important two component regulatory system, which regulates about 15{\%} of the gene expression in Streptococcus pyogenes. The covR/S locus was identified as an operon generating an RNA transcript around 2.5-kb in size. In this study, we found the covR/S operon produced three RNA transcripts (around 2.5-, 1.0-, and 0.8-kb in size). Using RNA transcriptional terminator sequence prediction and transcriptional terminator analysis, we identified two atypical rho-independent terminator sequences downstream of the covR gene and showed these terminator sequences terminate RNA transcription efficiently. These results indicate that covR/S operon generates covR/S transcript and monocistronic covR transcripts.",
author = "Chuan Chiang-Ni and Tsou, {Chih Cheng} and Yee-Shin Lin and Woei-Jer Chuang and Lin, {Ming T.} and Ching-Chuan Liu and Wu, {Jiunn Jong}",
year = "2008",
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language = "English",
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The transcriptional terminator sequences downstream of the covR gene terminate covR/S operon transcription to generate covR monocistronic transcripts in Streptococcus pyogenes. / Chiang-Ni, Chuan; Tsou, Chih Cheng; Lin, Yee-Shin; Chuang, Woei-Jer; Lin, Ming T.; Liu, Ching-Chuan; Wu, Jiunn Jong.

In: Gene, Vol. 427, No. 1-2, 31.12.2008, p. 99-103.

Research output: Contribution to journalArticle

TY - JOUR

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AU - Chiang-Ni, Chuan

AU - Tsou, Chih Cheng

AU - Lin, Yee-Shin

AU - Chuang, Woei-Jer

AU - Lin, Ming T.

AU - Liu, Ching-Chuan

AU - Wu, Jiunn Jong

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AB - CovR/S is an important two component regulatory system, which regulates about 15% of the gene expression in Streptococcus pyogenes. The covR/S locus was identified as an operon generating an RNA transcript around 2.5-kb in size. In this study, we found the covR/S operon produced three RNA transcripts (around 2.5-, 1.0-, and 0.8-kb in size). Using RNA transcriptional terminator sequence prediction and transcriptional terminator analysis, we identified two atypical rho-independent terminator sequences downstream of the covR gene and showed these terminator sequences terminate RNA transcription efficiently. These results indicate that covR/S operon generates covR/S transcript and monocistronic covR transcripts.

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