TY - JOUR
T1 - Thermal control of micro reverse transcription-polymerase chain reaction systems
AU - Tsai, Nan Chyuan
AU - Sue, Chung Yang
N1 - Funding Information:
The authors would like to thank the Center for Micro/Nano Technology Research, National Cheng Kung University, Tainan, Taiwan, and National Nano Devices Laboratory (NDL) for equipment access and technical support. This research was partially supported by National Science Council (Taiwan) with Grant NSC94-2212-E-006-054.
PY - 2007/5/1
Y1 - 2007/5/1
N2 - Being beneficial from dramatic progress in biochemical and micro-electromechanical technologies, traditional DNA manipulation devices tend to be miniaturized to speed up detection processing. Polymerase chain reaction (PCR) and reverse transcription-polymerase chain reaction (RT-PCR) are two typical examples of them. In this report, micro RT-PCR (μRT-PCR) is designed to quantitatively detect viruses. Test samples reservoirs, RT-PCR, and capillary electrophoresis are integrated on a SU-8 based monolithic chip. A high-precision temperature control unit is well developed by embedding amplification circuits and an Intel 8051 microprocessor. The integrated system exhibits high efficacy of heat transfer, exemption of fluid flow clogging and adequate sensitivity to, by feedback, control the cycle of detection for the infected malignant tissues via intensive simulations.
AB - Being beneficial from dramatic progress in biochemical and micro-electromechanical technologies, traditional DNA manipulation devices tend to be miniaturized to speed up detection processing. Polymerase chain reaction (PCR) and reverse transcription-polymerase chain reaction (RT-PCR) are two typical examples of them. In this report, micro RT-PCR (μRT-PCR) is designed to quantitatively detect viruses. Test samples reservoirs, RT-PCR, and capillary electrophoresis are integrated on a SU-8 based monolithic chip. A high-precision temperature control unit is well developed by embedding amplification circuits and an Intel 8051 microprocessor. The integrated system exhibits high efficacy of heat transfer, exemption of fluid flow clogging and adequate sensitivity to, by feedback, control the cycle of detection for the infected malignant tissues via intensive simulations.
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U2 - 10.1016/j.sna.2006.10.057
DO - 10.1016/j.sna.2006.10.057
M3 - Article
AN - SCOPUS:34247109456
SN - 0924-4247
VL - 136
SP - 178
EP - 183
JO - Sensors and Actuators, A: Physical
JF - Sensors and Actuators, A: Physical
IS - 1
ER -