Three-dimensional focusing for microflow cytometer with sequence micro-weir structures

Ho Cheng Lee, Che Hsin Lin, Lung-Ming Fu, Chien Hsiung Tsai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Micro flow cytometer has been widely used for counting and identifying individual cells. This study presents a novel microfluidic device for arranging cells/particles to a single file in the vertical direction for obtaining a great spatial resolution of signal detection in a micro-flow cytometric chip. Using the isotropic wet etching process and the photomask design, a sequence of micro-weir structures with cascaded height was fabricated on both top and bottom of the micro-fluidic channel. The experimental result shows that the fluorescence-labeled particles with the diameters of 7 and 15 m can be successfully discriminated using this proposed cascaded microweirs. The calculated coefficients of variation for the signal distribution of these two particles are 29.4% and 14.6%, respectively, indicating significantly enhances the detection performance. Unlike the conventional focusing techniques in vertical direction, the proposed cascaded micro-weirs can provide a simple yet effective flow focusing in vertical direction. The particles of different sizes can be validly arranged to a single file for providing a high-precision counting and discrimination capacity in micro-flow cytometric detection.

Original languageEnglish
Title of host publicationNEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
Pages698-702
Number of pages5
DOIs
Publication statusPublished - 2011 Oct 4
Event6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011 - Kaohsiung, Taiwan
Duration: 2011 Feb 202011 Feb 23

Other

Other6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011
CountryTaiwan
CityKaohsiung
Period11-02-2011-02-23

Fingerprint

microstructure
files
counting
photomasks
microfluidic devices
signal detection
fluidics
cells
discrimination
spatial resolution
chips
etching
fluorescence
coefficients

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

Cite this

Lee, H. C., Lin, C. H., Fu, L-M., & Tsai, C. H. (2011). Three-dimensional focusing for microflow cytometer with sequence micro-weir structures. In NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (pp. 698-702). [6017450] https://doi.org/10.1109/NEMS.2011.6017450
Lee, Ho Cheng ; Lin, Che Hsin ; Fu, Lung-Ming ; Tsai, Chien Hsiung. / Three-dimensional focusing for microflow cytometer with sequence micro-weir structures. NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems. 2011. pp. 698-702
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Lee, HC, Lin, CH, Fu, L-M & Tsai, CH 2011, Three-dimensional focusing for microflow cytometer with sequence micro-weir structures. in NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems., 6017450, pp. 698-702, 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011, Kaohsiung, Taiwan, 11-02-20. https://doi.org/10.1109/NEMS.2011.6017450

Three-dimensional focusing for microflow cytometer with sequence micro-weir structures. / Lee, Ho Cheng; Lin, Che Hsin; Fu, Lung-Ming; Tsai, Chien Hsiung.

NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems. 2011. p. 698-702 6017450.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Lee HC, Lin CH, Fu L-M, Tsai CH. Three-dimensional focusing for microflow cytometer with sequence micro-weir structures. In NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems. 2011. p. 698-702. 6017450 https://doi.org/10.1109/NEMS.2011.6017450