Three-dimensional focusing for microflow cytometer with sequence micro-weir structures

Ho Cheng Lee; Che Hsin Lin; Lung-Ming Fu; Chien Hsiung Tsai

doi:10.1109/NEMS.2011.6017450

Three-dimensional focusing for microflow cytometer with sequence micro-weir structures

Ho Cheng Lee, Che Hsin Lin, Lung-Ming Fu, Chien Hsiung Tsai

Department of Engineering Science

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Micro flow cytometer has been widely used for counting and identifying individual cells. This study presents a novel microfluidic device for arranging cells/particles to a single file in the vertical direction for obtaining a great spatial resolution of signal detection in a micro-flow cytometric chip. Using the isotropic wet etching process and the photomask design, a sequence of micro-weir structures with cascaded height was fabricated on both top and bottom of the micro-fluidic channel. The experimental result shows that the fluorescence-labeled particles with the diameters of 7 and 15 m can be successfully discriminated using this proposed cascaded microweirs. The calculated coefficients of variation for the signal distribution of these two particles are 29.4% and 14.6%, respectively, indicating significantly enhances the detection performance. Unlike the conventional focusing techniques in vertical direction, the proposed cascaded micro-weirs can provide a simple yet effective flow focusing in vertical direction. The particles of different sizes can be validly arranged to a single file for providing a high-precision counting and discrimination capacity in micro-flow cytometric detection.

Original language	English
Title of host publication	NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
Pages	698-702
Number of pages	5
DOIs	https://doi.org/10.1109/NEMS.2011.6017450
Publication status	Published - 2011 Oct 4
Event	6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011 - Kaohsiung, Taiwan Duration: 2011 Feb 20 → 2011 Feb 23

Other

Other	6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011
Country	Taiwan
City	Kaohsiung
Period	11-02-20 → 11-02-23

All Science Journal Classification (ASJC) codes

Atomic and Molecular Physics, and Optics

Access to Document

10.1109/NEMS.2011.6017450

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Cite this

@inproceedings{165a0a70a93840a7ad87c5f5dbd5fb99,

title = "Three-dimensional focusing for microflow cytometer with sequence micro-weir structures",

abstract = "Micro flow cytometer has been widely used for counting and identifying individual cells. This study presents a novel microfluidic device for arranging cells/particles to a single file in the vertical direction for obtaining a great spatial resolution of signal detection in a micro-flow cytometric chip. Using the isotropic wet etching process and the photomask design, a sequence of micro-weir structures with cascaded height was fabricated on both top and bottom of the micro-fluidic channel. The experimental result shows that the fluorescence-labeled particles with the diameters of 7 and 15 m can be successfully discriminated using this proposed cascaded microweirs. The calculated coefficients of variation for the signal distribution of these two particles are 29.4% and 14.6%, respectively, indicating significantly enhances the detection performance. Unlike the conventional focusing techniques in vertical direction, the proposed cascaded micro-weirs can provide a simple yet effective flow focusing in vertical direction. The particles of different sizes can be validly arranged to a single file for providing a high-precision counting and discrimination capacity in micro-flow cytometric detection.",

author = "Lee, {Ho Cheng} and Lin, {Che Hsin} and Lung-Ming Fu and Tsai, {Chien Hsiung}",

year = "2011",

month = oct,

day = "4",

doi = "10.1109/NEMS.2011.6017450",

language = "English",

isbn = "9781612847757",

pages = "698--702",

booktitle = "NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems",

note = "6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011 ; Conference date: 20-02-2011 Through 23-02-2011",

}

Lee, HC, Lin, CH, Fu, L-M & Tsai, CH 2011, Three-dimensional focusing for microflow cytometer with sequence micro-weir structures. in NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems., 6017450, pp. 698-702, 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011, Kaohsiung, Taiwan, 11-02-20. https://doi.org/10.1109/NEMS.2011.6017450

TY - GEN

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AU - Lee, Ho Cheng

AU - Lin, Che Hsin

AU - Fu, Lung-Ming

AU - Tsai, Chien Hsiung

PY - 2011/10/4

Y1 - 2011/10/4

N2 - Micro flow cytometer has been widely used for counting and identifying individual cells. This study presents a novel microfluidic device for arranging cells/particles to a single file in the vertical direction for obtaining a great spatial resolution of signal detection in a micro-flow cytometric chip. Using the isotropic wet etching process and the photomask design, a sequence of micro-weir structures with cascaded height was fabricated on both top and bottom of the micro-fluidic channel. The experimental result shows that the fluorescence-labeled particles with the diameters of 7 and 15 m can be successfully discriminated using this proposed cascaded microweirs. The calculated coefficients of variation for the signal distribution of these two particles are 29.4% and 14.6%, respectively, indicating significantly enhances the detection performance. Unlike the conventional focusing techniques in vertical direction, the proposed cascaded micro-weirs can provide a simple yet effective flow focusing in vertical direction. The particles of different sizes can be validly arranged to a single file for providing a high-precision counting and discrimination capacity in micro-flow cytometric detection.

AB - Micro flow cytometer has been widely used for counting and identifying individual cells. This study presents a novel microfluidic device for arranging cells/particles to a single file in the vertical direction for obtaining a great spatial resolution of signal detection in a micro-flow cytometric chip. Using the isotropic wet etching process and the photomask design, a sequence of micro-weir structures with cascaded height was fabricated on both top and bottom of the micro-fluidic channel. The experimental result shows that the fluorescence-labeled particles with the diameters of 7 and 15 m can be successfully discriminated using this proposed cascaded microweirs. The calculated coefficients of variation for the signal distribution of these two particles are 29.4% and 14.6%, respectively, indicating significantly enhances the detection performance. Unlike the conventional focusing techniques in vertical direction, the proposed cascaded micro-weirs can provide a simple yet effective flow focusing in vertical direction. The particles of different sizes can be validly arranged to a single file for providing a high-precision counting and discrimination capacity in micro-flow cytometric detection.

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