Abstract
Micro flow cytometer has been widely used for counting and identifying individual cells. This study presents a novel microfluidic device for arranging cells/particles to a single file in the vertical direction for obtaining a great spatial resolution of signal detection in a micro-flow cytometric chip. Using the isotropic wet etching process and the photomask design, a sequence of micro-weir structures with cascaded height was fabricated on both top and bottom of the micro-fluidic channel. The experimental result shows that the fluorescence-labeled particles with the diameters of 7 and 15 m can be successfully discriminated using this proposed cascaded microweirs. The calculated coefficients of variation for the signal distribution of these two particles are 29.4% and 14.6%, respectively, indicating significantly enhances the detection performance. Unlike the conventional focusing techniques in vertical direction, the proposed cascaded micro-weirs can provide a simple yet effective flow focusing in vertical direction. The particles of different sizes can be validly arranged to a single file for providing a high-precision counting and discrimination capacity in micro-flow cytometric detection.
| Original language | English |
|---|---|
| Title of host publication | NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems |
| Pages | 698-702 |
| Number of pages | 5 |
| DOIs | |
| Publication status | Published - 2011 Oct 4 |
| Event | 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011 - Kaohsiung, Taiwan Duration: 2011 Feb 20 → 2011 Feb 23 |
Other
| Other | 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011 |
|---|---|
| Country | Taiwan |
| City | Kaohsiung |
| Period | 11-02-20 → 11-02-23 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Atomic and Molecular Physics, and Optics
Cite this
}
Three-dimensional focusing for microflow cytometer with sequence micro-weir structures. / Lee, Ho Cheng; Lin, Che Hsin; Fu, Lung-Ming; Tsai, Chien Hsiung.
NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems. 2011. p. 698-702 6017450.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - Three-dimensional focusing for microflow cytometer with sequence micro-weir structures
AU - Lee, Ho Cheng
AU - Lin, Che Hsin
AU - Fu, Lung-Ming
AU - Tsai, Chien Hsiung
PY - 2011/10/4
Y1 - 2011/10/4
N2 - Micro flow cytometer has been widely used for counting and identifying individual cells. This study presents a novel microfluidic device for arranging cells/particles to a single file in the vertical direction for obtaining a great spatial resolution of signal detection in a micro-flow cytometric chip. Using the isotropic wet etching process and the photomask design, a sequence of micro-weir structures with cascaded height was fabricated on both top and bottom of the micro-fluidic channel. The experimental result shows that the fluorescence-labeled particles with the diameters of 7 and 15 m can be successfully discriminated using this proposed cascaded microweirs. The calculated coefficients of variation for the signal distribution of these two particles are 29.4% and 14.6%, respectively, indicating significantly enhances the detection performance. Unlike the conventional focusing techniques in vertical direction, the proposed cascaded micro-weirs can provide a simple yet effective flow focusing in vertical direction. The particles of different sizes can be validly arranged to a single file for providing a high-precision counting and discrimination capacity in micro-flow cytometric detection.
AB - Micro flow cytometer has been widely used for counting and identifying individual cells. This study presents a novel microfluidic device for arranging cells/particles to a single file in the vertical direction for obtaining a great spatial resolution of signal detection in a micro-flow cytometric chip. Using the isotropic wet etching process and the photomask design, a sequence of micro-weir structures with cascaded height was fabricated on both top and bottom of the micro-fluidic channel. The experimental result shows that the fluorescence-labeled particles with the diameters of 7 and 15 m can be successfully discriminated using this proposed cascaded microweirs. The calculated coefficients of variation for the signal distribution of these two particles are 29.4% and 14.6%, respectively, indicating significantly enhances the detection performance. Unlike the conventional focusing techniques in vertical direction, the proposed cascaded micro-weirs can provide a simple yet effective flow focusing in vertical direction. The particles of different sizes can be validly arranged to a single file for providing a high-precision counting and discrimination capacity in micro-flow cytometric detection.
UR - http://www.scopus.com/inward/record.url?scp=80053334642&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80053334642&partnerID=8YFLogxK
U2 - 10.1109/NEMS.2011.6017450
DO - 10.1109/NEMS.2011.6017450
M3 - Conference contribution
AN - SCOPUS:80053334642
SN - 9781612847757
SP - 698
EP - 702
BT - NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
ER -