Thrombomodulin promotes corneal epithelial wound healing

Yi Hsun Huang, I. Ching-Chang, Cheng Hsiang Kuo, Yun Yan Hsu, Fang Tzu Lee, Guey Yueh Shi, Sung Huei Tseng, Hua Lin Wu

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


Purpose: To determine the role of thrombomodulin (TM) in corneal epithelial wound healing, and to investigate whether recombinant TM epidermal growth factor-like domain plus serine/threoninerich domain (rTMD23) has therapeutic potential in corneal epithelial wound healing. Methods: TM localization and expression in the murine cornea were examined by immunofluorescence staining. TM expression after injury was also studied. The effect of rTMD23 on corneal wound healing was evaluated by in vitro and in vivo assays. Results: TM was expressed in the cornea in normal adult mice. TM expression increased in the early phase of wound healing and decreased after wound recovery. In the in vitro study, plateletderived growth factor-BB (PDGF-BB) induced TM expression in murine corneal epithelial cells by mediating E26 transformation-specific sequence-1 (Ets-1) via the mammalian target of rapamycin (mTOR) signaling pathway. The administration of rTMD23 increased the rate of corneal epithelial wound healing. Conclusions: TM expression in corneal epithelium was modulated during the corneal wound healing process, and may be regulated by PDGF-BB. In addition, rTMD23 has therapeutic potential in corneal injury.

Original languageEnglish
Article numbere0122491
JournalPloS one
Issue number3
Publication statusPublished - 2015 Mar 27

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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