Tic40 is important for reinsertion of proteins from the chloroplast stroma into the inner membrane

Chi-Chou Chiu, Hsou Min Li

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Chloroplast inner-membrane proteins Tic40 and Tic110 are first imported from the cytosol into the chloroplast stroma, and subsequently reinserted from the stroma into the inner membrane. However, the mechanism of reinsertion remains unclear. Here we show that Tic40 itself is involved in this reinsertion process. When precursors of either Tic40 or a Tic110 C-terminal truncate, tpTic110-Tic110N, were imported into chloroplasts isolated from a tic40-null mutant, soluble Tic40 and Tic110N intermediates accumulated in the stroma of tic40-mutant chloroplasts, due to a slower rate of reinsertion. We further show that a larger quantity of soluble Tic21 intermediates also accumulated in the stroma of tic40-mutant chloroplasts. In contrast, inner-membrane insertion of the triose-phosphate/phosphate translocator was not affected by the tic40 mutation. Our data suggest that multiple pathways exist for the insertion of chloroplast inner-membrane proteins.

Original languageEnglish
Pages (from-to)793-801
Number of pages9
JournalPlant Journal
Volume56
Issue number5
DOIs
Publication statusPublished - 2008 Dec 1

Fingerprint

Chloroplast Proteins
Chloroplasts
chloroplasts
Membranes
proteins
membrane proteins
mutants
Membrane Proteins
Trioses
Phosphates
phosphates
trioses
cytosol
Cytosol
mutation
Mutation

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

@article{0552b8b24c0d4a089f898a0aa96556be,
title = "Tic40 is important for reinsertion of proteins from the chloroplast stroma into the inner membrane",
abstract = "Chloroplast inner-membrane proteins Tic40 and Tic110 are first imported from the cytosol into the chloroplast stroma, and subsequently reinserted from the stroma into the inner membrane. However, the mechanism of reinsertion remains unclear. Here we show that Tic40 itself is involved in this reinsertion process. When precursors of either Tic40 or a Tic110 C-terminal truncate, tpTic110-Tic110N, were imported into chloroplasts isolated from a tic40-null mutant, soluble Tic40 and Tic110N intermediates accumulated in the stroma of tic40-mutant chloroplasts, due to a slower rate of reinsertion. We further show that a larger quantity of soluble Tic21 intermediates also accumulated in the stroma of tic40-mutant chloroplasts. In contrast, inner-membrane insertion of the triose-phosphate/phosphate translocator was not affected by the tic40 mutation. Our data suggest that multiple pathways exist for the insertion of chloroplast inner-membrane proteins.",
author = "Chi-Chou Chiu and Li, {Hsou Min}",
year = "2008",
month = "12",
day = "1",
doi = "10.1111/j.1365-313X.2008.03638.x",
language = "English",
volume = "56",
pages = "793--801",
journal = "Plant Journal",
issn = "0960-7412",
publisher = "Wiley-Blackwell",
number = "5",

}

Tic40 is important for reinsertion of proteins from the chloroplast stroma into the inner membrane. / Chiu, Chi-Chou; Li, Hsou Min.

In: Plant Journal, Vol. 56, No. 5, 01.12.2008, p. 793-801.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Tic40 is important for reinsertion of proteins from the chloroplast stroma into the inner membrane

AU - Chiu, Chi-Chou

AU - Li, Hsou Min

PY - 2008/12/1

Y1 - 2008/12/1

N2 - Chloroplast inner-membrane proteins Tic40 and Tic110 are first imported from the cytosol into the chloroplast stroma, and subsequently reinserted from the stroma into the inner membrane. However, the mechanism of reinsertion remains unclear. Here we show that Tic40 itself is involved in this reinsertion process. When precursors of either Tic40 or a Tic110 C-terminal truncate, tpTic110-Tic110N, were imported into chloroplasts isolated from a tic40-null mutant, soluble Tic40 and Tic110N intermediates accumulated in the stroma of tic40-mutant chloroplasts, due to a slower rate of reinsertion. We further show that a larger quantity of soluble Tic21 intermediates also accumulated in the stroma of tic40-mutant chloroplasts. In contrast, inner-membrane insertion of the triose-phosphate/phosphate translocator was not affected by the tic40 mutation. Our data suggest that multiple pathways exist for the insertion of chloroplast inner-membrane proteins.

AB - Chloroplast inner-membrane proteins Tic40 and Tic110 are first imported from the cytosol into the chloroplast stroma, and subsequently reinserted from the stroma into the inner membrane. However, the mechanism of reinsertion remains unclear. Here we show that Tic40 itself is involved in this reinsertion process. When precursors of either Tic40 or a Tic110 C-terminal truncate, tpTic110-Tic110N, were imported into chloroplasts isolated from a tic40-null mutant, soluble Tic40 and Tic110N intermediates accumulated in the stroma of tic40-mutant chloroplasts, due to a slower rate of reinsertion. We further show that a larger quantity of soluble Tic21 intermediates also accumulated in the stroma of tic40-mutant chloroplasts. In contrast, inner-membrane insertion of the triose-phosphate/phosphate translocator was not affected by the tic40 mutation. Our data suggest that multiple pathways exist for the insertion of chloroplast inner-membrane proteins.

UR - http://www.scopus.com/inward/record.url?scp=57449097581&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=57449097581&partnerID=8YFLogxK

U2 - 10.1111/j.1365-313X.2008.03638.x

DO - 10.1111/j.1365-313X.2008.03638.x

M3 - Article

C2 - 18657235

AN - SCOPUS:57449097581

VL - 56

SP - 793

EP - 801

JO - Plant Journal

JF - Plant Journal

SN - 0960-7412

IS - 5

ER -