Tic40 is important for reinsertion of proteins from the chloroplast stroma into the inner membrane

Chi Chou Chiu, Hsou Min Li

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

Chloroplast inner-membrane proteins Tic40 and Tic110 are first imported from the cytosol into the chloroplast stroma, and subsequently reinserted from the stroma into the inner membrane. However, the mechanism of reinsertion remains unclear. Here we show that Tic40 itself is involved in this reinsertion process. When precursors of either Tic40 or a Tic110 C-terminal truncate, tpTic110-Tic110N, were imported into chloroplasts isolated from a tic40-null mutant, soluble Tic40 and Tic110N intermediates accumulated in the stroma of tic40-mutant chloroplasts, due to a slower rate of reinsertion. We further show that a larger quantity of soluble Tic21 intermediates also accumulated in the stroma of tic40-mutant chloroplasts. In contrast, inner-membrane insertion of the triose-phosphate/phosphate translocator was not affected by the tic40 mutation. Our data suggest that multiple pathways exist for the insertion of chloroplast inner-membrane proteins.

Original languageEnglish
Pages (from-to)793-801
Number of pages9
JournalPlant Journal
Volume56
Issue number5
DOIs
Publication statusPublished - 2008 Dec

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science
  • Cell Biology

Fingerprint Dive into the research topics of 'Tic40 is important for reinsertion of proteins from the chloroplast stroma into the inner membrane'. Together they form a unique fingerprint.

Cite this