Toll-like receptor 4 plays an anti-HBV role in a murine model of acute hepatitis B virus expression

Wen Wei Chang, Ih Jen Su, Ming-Derg Lai, Wen-Tsan Chang, Wen-Ya Huang, Huan Yao Lei

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Aim: Toll-like receptor 4 (TLR4) has been shown to be important for bacterial infection, especially to lipopolysaccharide signaling. Its possible role in HBV infection is studied in the present study. Materials and methods: pHBV3.6 plasmid, containing full-length HBV genome was used in the murine model of acute HBV expression by hydrodynamics in vivo transfection. TLR4 normal or mutant mouse strain was compared to investigate the possible role of TLR4 in acute HBV expression. Results: After pHBV3.6 injection, the infiltrating leukocytes expressed TLR4 were observed nearby the HBsAg-expressing hepatocytes. The HBV antigenemia as well as the replication and transcription were higher in TLR4-mutant C3H/HeJ mice than in normal C3H/HeN mice. The HBV-specific immune responses were impaired in the liver or spleen of the C3H/HeJ mice. Their inducible nitric oxide synthase (iNOS) expression on the hepatic infiltrating cells was also impaired. When adoptively transferring splenocytes from C3H/HeN mice to C3H/HeJ mice, the HBV replication was inhibited to the level as that of C3H/HeN. Conclusion: These results suggest that TLR4 plays an anti-HBV role in vivo through the induction of iNOS expression and HBV-specific immune responses after HBV expression.

Original languageEnglish
Pages (from-to)6631-6637
Number of pages7
JournalWorld journal of gastroenterology
Volume11
Issue number42
DOIs
Publication statusPublished - 2005 Nov 14

Fingerprint

Toll-Like Receptor 4
Hepatitis B virus
Inbred C3H Mouse
Nitric Oxide Synthase Type II
Hepatocytes
Mutant Strains Mice
Hydrodynamics
Hepatitis B Surface Antigens
Bacterial Infections
Transfection
Lipopolysaccharides
Leukocytes
Plasmids
Spleen
Genome
Injections
Liver
Infection

All Science Journal Classification (ASJC) codes

  • Gastroenterology

Cite this

@article{f1ce82fdb1c84c66a52771c8f9d62fc6,
title = "Toll-like receptor 4 plays an anti-HBV role in a murine model of acute hepatitis B virus expression",
abstract = "Aim: Toll-like receptor 4 (TLR4) has been shown to be important for bacterial infection, especially to lipopolysaccharide signaling. Its possible role in HBV infection is studied in the present study. Materials and methods: pHBV3.6 plasmid, containing full-length HBV genome was used in the murine model of acute HBV expression by hydrodynamics in vivo transfection. TLR4 normal or mutant mouse strain was compared to investigate the possible role of TLR4 in acute HBV expression. Results: After pHBV3.6 injection, the infiltrating leukocytes expressed TLR4 were observed nearby the HBsAg-expressing hepatocytes. The HBV antigenemia as well as the replication and transcription were higher in TLR4-mutant C3H/HeJ mice than in normal C3H/HeN mice. The HBV-specific immune responses were impaired in the liver or spleen of the C3H/HeJ mice. Their inducible nitric oxide synthase (iNOS) expression on the hepatic infiltrating cells was also impaired. When adoptively transferring splenocytes from C3H/HeN mice to C3H/HeJ mice, the HBV replication was inhibited to the level as that of C3H/HeN. Conclusion: These results suggest that TLR4 plays an anti-HBV role in vivo through the induction of iNOS expression and HBV-specific immune responses after HBV expression.",
author = "Chang, {Wen Wei} and Su, {Ih Jen} and Ming-Derg Lai and Wen-Tsan Chang and Wen-Ya Huang and Lei, {Huan Yao}",
year = "2005",
month = "11",
day = "14",
doi = "10.3748/wjg.v11.i42.6631",
language = "English",
volume = "11",
pages = "6631--6637",
journal = "World Journal of Gastroenterology",
issn = "1007-9327",
publisher = "WJG Press",
number = "42",

}

Toll-like receptor 4 plays an anti-HBV role in a murine model of acute hepatitis B virus expression. / Chang, Wen Wei; Su, Ih Jen; Lai, Ming-Derg; Chang, Wen-Tsan; Huang, Wen-Ya; Lei, Huan Yao.

In: World journal of gastroenterology, Vol. 11, No. 42, 14.11.2005, p. 6631-6637.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Toll-like receptor 4 plays an anti-HBV role in a murine model of acute hepatitis B virus expression

AU - Chang, Wen Wei

AU - Su, Ih Jen

AU - Lai, Ming-Derg

AU - Chang, Wen-Tsan

AU - Huang, Wen-Ya

AU - Lei, Huan Yao

PY - 2005/11/14

Y1 - 2005/11/14

N2 - Aim: Toll-like receptor 4 (TLR4) has been shown to be important for bacterial infection, especially to lipopolysaccharide signaling. Its possible role in HBV infection is studied in the present study. Materials and methods: pHBV3.6 plasmid, containing full-length HBV genome was used in the murine model of acute HBV expression by hydrodynamics in vivo transfection. TLR4 normal or mutant mouse strain was compared to investigate the possible role of TLR4 in acute HBV expression. Results: After pHBV3.6 injection, the infiltrating leukocytes expressed TLR4 were observed nearby the HBsAg-expressing hepatocytes. The HBV antigenemia as well as the replication and transcription were higher in TLR4-mutant C3H/HeJ mice than in normal C3H/HeN mice. The HBV-specific immune responses were impaired in the liver or spleen of the C3H/HeJ mice. Their inducible nitric oxide synthase (iNOS) expression on the hepatic infiltrating cells was also impaired. When adoptively transferring splenocytes from C3H/HeN mice to C3H/HeJ mice, the HBV replication was inhibited to the level as that of C3H/HeN. Conclusion: These results suggest that TLR4 plays an anti-HBV role in vivo through the induction of iNOS expression and HBV-specific immune responses after HBV expression.

AB - Aim: Toll-like receptor 4 (TLR4) has been shown to be important for bacterial infection, especially to lipopolysaccharide signaling. Its possible role in HBV infection is studied in the present study. Materials and methods: pHBV3.6 plasmid, containing full-length HBV genome was used in the murine model of acute HBV expression by hydrodynamics in vivo transfection. TLR4 normal or mutant mouse strain was compared to investigate the possible role of TLR4 in acute HBV expression. Results: After pHBV3.6 injection, the infiltrating leukocytes expressed TLR4 were observed nearby the HBsAg-expressing hepatocytes. The HBV antigenemia as well as the replication and transcription were higher in TLR4-mutant C3H/HeJ mice than in normal C3H/HeN mice. The HBV-specific immune responses were impaired in the liver or spleen of the C3H/HeJ mice. Their inducible nitric oxide synthase (iNOS) expression on the hepatic infiltrating cells was also impaired. When adoptively transferring splenocytes from C3H/HeN mice to C3H/HeJ mice, the HBV replication was inhibited to the level as that of C3H/HeN. Conclusion: These results suggest that TLR4 plays an anti-HBV role in vivo through the induction of iNOS expression and HBV-specific immune responses after HBV expression.

UR - http://www.scopus.com/inward/record.url?scp=30444436486&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=30444436486&partnerID=8YFLogxK

U2 - 10.3748/wjg.v11.i42.6631

DO - 10.3748/wjg.v11.i42.6631

M3 - Article

VL - 11

SP - 6631

EP - 6637

JO - World Journal of Gastroenterology

JF - World Journal of Gastroenterology

SN - 1007-9327

IS - 42

ER -