Topical exposure to carbon disulfide induces epidermal permeability alterations in physiological and pathological changes

Tzu Chieh Chou, Jui Chen Tsai, Hamm Min Sheu, Chauying J. Jen, Tung Sheng Shih, Ho Yuan Chang

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Carbon disulfide (CS2) has been suggested its possible skin toxicity. Neither a dose-response relationship nor any mechanism of CS 2-exposure regarding epidermal permeability alterations has been postulated. The objectives of this study were to evaluate the dose-dependent association and the pathological changes with CS2 topically applied to mouse epidermis. Four concentrations of CS2 (0% (controls), 10%, 15%, and 20% in ethanol) were topically applied to a 1.8 cm2 area of the lateral abdomen of female nude mice for 10 min. Time-series transepidermal water loss (TEWL) profile, morphological examinations by both light microscopy (hematoxylin/eosin stain and Nile Red stain) and electronic microscopy, and lipid analysis by high performance thin-layer chromatography (HPTLC) were used to evaluate the epidermal impairment. We found no recovery occurred within 72 h exposure to 20% CS2 in contrast to substantial recovery found in 10% and 15% CS2-exposure. Clear dose-dependent fashions were shown in TEWL elevations, recovery retardation, and lipid extraction across the ethanol (control), 10%, 15%, and 20% CS2 exposures. Two mechanistic pathways were raised to account for CS2-induced epidermal alterations: intercellular lipid depletion and keratinocyte damage. A study with different test animal species is warranted owing to the discrepancies in epidermis between nude mice and other species.

Original languageEnglish
Pages (from-to)225-236
Number of pages12
JournalToxicology Letters
Volume158
Issue number3
DOIs
Publication statusPublished - 2005 Sep 15

All Science Journal Classification (ASJC) codes

  • Toxicology

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