Toward reliable retrieval of functional information of papillary dermis using spatially resolved diffuse reflectance spectroscopy

Yu Wen Chen, Jun Yen Guo, Shih Yu Tzeng, Ting Chun Chou, Ming Jen Lin, Lynn Ling Huei Huang, Chao Chun Yang, Chao Kai Hsu, Sheng Hao Tseng

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Spatially resolved diffuse reflectance spectroscopy (SRDRS) has been employed to quantify tissue optical properties and its interrogation volume is majorly controlled by the source-to-detector separations (SDSs). To noninvasively quantify properties of dermis, a SRDRS setup that includes SDS shorter than 1 mm is required. It will be demonstrated in this study that Monte Carlo simulations employing the Henyey-Greenstein phase function cannot always precisely predict experimentally measured diffuse reflectance at such short SDSs, and we speculated this could be caused by the non-negligible backward light scattering at short SDSs that cannot be properly modeled by the Henyey-Greenstein phase function. To accurately recover the optical properties and functional information of dermis using SRDRS, we proposed the use of the modified two-layer (MTL) geometry. Monte Carlo simulations and phantom experiment results revealed that the MTL probing geometry was capable of faithfully recovering the optical properties of upper dermis. The capability of the MTL geometry in probing the upper dermis properties was further verified through a swine study, and it was found that the measurement results were reasonably linked to histological findings. Finally, the MTL probe was utilized to study psoriatic lesions. Our results showed that the MTL probe was sensitive to the physiological condition of tissue volumes within the papillary dermis and could be used in studying the physiology of psoriasis.

Original languageEnglish
Article number252734
Pages (from-to)542-558
Number of pages17
JournalBiomedical Optics Express
Volume7
Issue number2
DOIs
Publication statusPublished - 2016 Jan 19

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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