Towards portable flow cytometry: Study on the use of air-sheath-based volume-efficient two-phase microfluidic systems

Dongeun Huh; Alan H. Tkaczyk; Hsien Hung Wei; James B. Grotberg; Shuichi Takayama

Towards portable flow cytometry: Study on the use of air-sheath-based volume-efficient two-phase microfluidic systems

Dongeun Huh, Alan H. Tkaczyk, Hsien Hung Wei, James B. Grotberg, Shuichi Takayama

Department of Chemical Engineering

Research output: Contribution to journal › Conference article › peer-review

4 Citations (Scopus)

Abstract

This paper reports experimental studies on air-liquid two-phase stratified flows in polymeric microfluidic channels for volume-efficient and cost-effective flow cytometry. Different flow behaviors of liquid core streams sheathed by air flows were investigated by varying chemistry and geometry of PDMS channels. Fluorescent beads and myoblast cells carried by stable liquid flows were analyzed by digital photo-multiplier tube (PMT)-based optical detection system to analyze the number of particles in a sample.

Original language	English
Pages (from-to)	1642-1643
Number of pages	2
Journal	Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume	2
Publication status	Published - 2002 Dec 1
Event	Proceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) - Houston, TX, United States Duration: 2002 Oct 23 → 2002 Oct 26

All Science Journal Classification (ASJC) codes

Signal Processing
Biomedical Engineering
Computer Vision and Pattern Recognition
Health Informatics

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title = "Towards portable flow cytometry: Study on the use of air-sheath-based volume-efficient two-phase microfluidic systems",

abstract = "This paper reports experimental studies on air-liquid two-phase stratified flows in polymeric microfluidic channels for volume-efficient and cost-effective flow cytometry. Different flow behaviors of liquid core streams sheathed by air flows were investigated by varying chemistry and geometry of PDMS channels. Fluorescent beads and myoblast cells carried by stable liquid flows were analyzed by digital photo-multiplier tube (PMT)-based optical detection system to analyze the number of particles in a sample.",

author = "Dongeun Huh and Tkaczyk, {Alan H.} and Wei, {Hsien Hung} and Grotberg, {James B.} and Shuichi Takayama",

year = "2002",

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journal = "Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings",

issn = "1557-170X",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

note = "Proceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) ; Conference date: 23-10-2002 Through 26-10-2002",

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Towards portable flow cytometry : Study on the use of air-sheath-based volume-efficient two-phase microfluidic systems. / Huh, Dongeun; Tkaczyk, Alan H.; Wei, Hsien Hung; Grotberg, James B.; Takayama, Shuichi.

In: Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings, Vol. 2, 01.12.2002, p. 1642-1643.

Research output: Contribution to journal › Conference article › peer-review

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T2 - Proceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS)

AU - Huh, Dongeun

AU - Tkaczyk, Alan H.

AU - Wei, Hsien Hung

AU - Grotberg, James B.

AU - Takayama, Shuichi

PY - 2002/12/1

Y1 - 2002/12/1

N2 - This paper reports experimental studies on air-liquid two-phase stratified flows in polymeric microfluidic channels for volume-efficient and cost-effective flow cytometry. Different flow behaviors of liquid core streams sheathed by air flows were investigated by varying chemistry and geometry of PDMS channels. Fluorescent beads and myoblast cells carried by stable liquid flows were analyzed by digital photo-multiplier tube (PMT)-based optical detection system to analyze the number of particles in a sample.

AB - This paper reports experimental studies on air-liquid two-phase stratified flows in polymeric microfluidic channels for volume-efficient and cost-effective flow cytometry. Different flow behaviors of liquid core streams sheathed by air flows were investigated by varying chemistry and geometry of PDMS channels. Fluorescent beads and myoblast cells carried by stable liquid flows were analyzed by digital photo-multiplier tube (PMT)-based optical detection system to analyze the number of particles in a sample.

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