Towards portable flow cytometry: Study on the use of air-sheath-based volume-efficient two-phase microfluidic systems

Dongeun Huh, Alan H. Tkaczyk, Hsien Hung Wei, James B. Grotberg, Shuichi Takayama

Research output: Contribution to journalConference article

4 Citations (Scopus)

Abstract

This paper reports experimental studies on air-liquid two-phase stratified flows in polymeric microfluidic channels for volume-efficient and cost-effective flow cytometry. Different flow behaviors of liquid core streams sheathed by air flows were investigated by varying chemistry and geometry of PDMS channels. Fluorescent beads and myoblast cells carried by stable liquid flows were analyzed by digital photo-multiplier tube (PMT)-based optical detection system to analyze the number of particles in a sample.

Original languageEnglish
Pages (from-to)1642-1643
Number of pages2
JournalAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume2
Publication statusPublished - 2002 Dec 1
EventProceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) - Houston, TX, United States
Duration: 2002 Oct 232002 Oct 26

Fingerprint

Microfluidics
Flow cytometry
Flow Cytometry
Air
Optical Devices
Myoblasts
Liquids
Costs and Cost Analysis
Geometry
Costs

All Science Journal Classification (ASJC) codes

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

Cite this

@article{ec3621792bdb4e8e9146a9dbbaf40a7b,
title = "Towards portable flow cytometry: Study on the use of air-sheath-based volume-efficient two-phase microfluidic systems",
abstract = "This paper reports experimental studies on air-liquid two-phase stratified flows in polymeric microfluidic channels for volume-efficient and cost-effective flow cytometry. Different flow behaviors of liquid core streams sheathed by air flows were investigated by varying chemistry and geometry of PDMS channels. Fluorescent beads and myoblast cells carried by stable liquid flows were analyzed by digital photo-multiplier tube (PMT)-based optical detection system to analyze the number of particles in a sample.",
author = "Dongeun Huh and Tkaczyk, {Alan H.} and Wei, {Hsien Hung} and Grotberg, {James B.} and Shuichi Takayama",
year = "2002",
month = "12",
day = "1",
language = "English",
volume = "2",
pages = "1642--1643",
journal = "Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings",
issn = "1557-170X",
publisher = "Institute of Electrical and Electronics Engineers Inc.",

}

Towards portable flow cytometry : Study on the use of air-sheath-based volume-efficient two-phase microfluidic systems. / Huh, Dongeun; Tkaczyk, Alan H.; Wei, Hsien Hung; Grotberg, James B.; Takayama, Shuichi.

In: Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings, Vol. 2, 01.12.2002, p. 1642-1643.

Research output: Contribution to journalConference article

TY - JOUR

T1 - Towards portable flow cytometry

T2 - Study on the use of air-sheath-based volume-efficient two-phase microfluidic systems

AU - Huh, Dongeun

AU - Tkaczyk, Alan H.

AU - Wei, Hsien Hung

AU - Grotberg, James B.

AU - Takayama, Shuichi

PY - 2002/12/1

Y1 - 2002/12/1

N2 - This paper reports experimental studies on air-liquid two-phase stratified flows in polymeric microfluidic channels for volume-efficient and cost-effective flow cytometry. Different flow behaviors of liquid core streams sheathed by air flows were investigated by varying chemistry and geometry of PDMS channels. Fluorescent beads and myoblast cells carried by stable liquid flows were analyzed by digital photo-multiplier tube (PMT)-based optical detection system to analyze the number of particles in a sample.

AB - This paper reports experimental studies on air-liquid two-phase stratified flows in polymeric microfluidic channels for volume-efficient and cost-effective flow cytometry. Different flow behaviors of liquid core streams sheathed by air flows were investigated by varying chemistry and geometry of PDMS channels. Fluorescent beads and myoblast cells carried by stable liquid flows were analyzed by digital photo-multiplier tube (PMT)-based optical detection system to analyze the number of particles in a sample.

UR - http://www.scopus.com/inward/record.url?scp=0036906813&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036906813&partnerID=8YFLogxK

M3 - Conference article

AN - SCOPUS:0036906813

VL - 2

SP - 1642

EP - 1643

JO - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings

JF - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings

SN - 1557-170X

ER -