TY - JOUR
T1 - Towards protein production and application by using Chlorella species as circular economy
AU - Lai, Yu Cheng
AU - Chang, Chien Hsiang
AU - Chen, Chun Yen
AU - Chang, Jo Shu
AU - Ng, I. Son
N1 - Funding Information:
The authors are grateful for the financial support received from the Ministry of Science and Technology (MOST 108-2218-E-006-006, MOST 107-2218-E-006-016, MOST 106-3114-E-006-008, MOST 105-2221-E-006-225-MY3 and MOST 105-2621-M-006-012-MY3) in Taiwan.
Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2019/10
Y1 - 2019/10
N2 - In this study, productions of microalgal proteins were explored via a circular economy concept. First, production of proteins from Chlorella vulgaris FSP-E (CV) and Chlorella sorokiniana (CS) was optimized by using favorable cultivation conditions and strategies. The optimal CO2 concentration for the growth of both microalgae was 5% (v/v), while the optimal nitrogen source for CV and CS were 12 mM of NaNO3 and NH4Cl, respectively. Addition of 12 mg/L ammonium iron (III) citrate enhanced protein production. Next, semi-batch cultivation strategy was employed to achieve a protein production of 793.3 and 812.8 mg/L for CV and C S, representing a 4.86 and 2.77 fold increase, respectively, in protein productivity. The obtained microalgal proteins consist of 40% essential amino acids. The CV and CS proteins possess prebiotic activities as they enhanced the growth of Lactobacillus rhamnosus ZY by 48 and 74%, respectively, with a good antibacterial activity against predominant pathogens.
AB - In this study, productions of microalgal proteins were explored via a circular economy concept. First, production of proteins from Chlorella vulgaris FSP-E (CV) and Chlorella sorokiniana (CS) was optimized by using favorable cultivation conditions and strategies. The optimal CO2 concentration for the growth of both microalgae was 5% (v/v), while the optimal nitrogen source for CV and CS were 12 mM of NaNO3 and NH4Cl, respectively. Addition of 12 mg/L ammonium iron (III) citrate enhanced protein production. Next, semi-batch cultivation strategy was employed to achieve a protein production of 793.3 and 812.8 mg/L for CV and C S, representing a 4.86 and 2.77 fold increase, respectively, in protein productivity. The obtained microalgal proteins consist of 40% essential amino acids. The CV and CS proteins possess prebiotic activities as they enhanced the growth of Lactobacillus rhamnosus ZY by 48 and 74%, respectively, with a good antibacterial activity against predominant pathogens.
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U2 - 10.1016/j.biortech.2019.121625
DO - 10.1016/j.biortech.2019.121625
M3 - Article
C2 - 31203183
AN - SCOPUS:85067201191
SN - 0960-8524
VL - 289
JO - Bioresource technology
JF - Bioresource technology
M1 - 121625
ER -
