Toxicity assessment of mono-substituted benzenes and phenols using a Pseudomonas initial oxygen uptake assay

Ded Shih Huang, Thou-Jen Whang, Fei Chen Cheng, Ya Ping Wu, Yi Ting Wang, Wen I. Luo, Yane Shih Wang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A methodology is presented for assessing the toxicity of chemical substances through their inhibitory action toward the Pseudomonas initial oxygen uptake (PIOU) rate. The current studies reveal that the PIOU assay is rapid, cost-efficient, and easy to perform. The oxygen uptake rate was found to be associated with a putative benzoate transporter and highly dependent on benzoate concentration. The putative benzoate transporter has been shown to follow Michaelis-Menten kinetics. Most phenols were found to be noncompetitive inhibitors of the benzoate transporter. The inhibition constant (Ki) of these noncompetitive inhibitors can be related to the concentration causing 50% oxygen uptake inhibition in Pseudomonas putida. Modeling these data by using the response-surface approach leads to the development of a quantitative structure-activity relationship (QSAR) for the toxicity of phenols ((1/K i) = -0.435 (±0.038) lowest-unoccupied-molecular orbital + 0.517 (±0.027)log KOW - 2.340 (±0.068), n = 49, r 2 = 0.930, s = 0.107, radj2 = 0.926, F = 303.1). A comparison of QSAR models derived from the Ki data of the PIOU method and the toxicity data of 40-h Tetrahymena pyrifomis growth inhibition assay (Tetratox) indicated that there was a high correlation between the two approaches (r2 = 0.925).

Original languageEnglish
Pages (from-to)253-260
Number of pages8
JournalEnvironmental Toxicology and Chemistry
Volume24
Issue number2
DOIs
Publication statusPublished - 2005 Feb 1

Fingerprint

Phenols
Pseudomonas
Benzene
benzene
Benzoates
Toxicity
phenol
Assays
assay
Oxygen
toxicity
oxygen
Quantitative Structure-Activity Relationship
inhibitor
Tetrahymena
chemical substance
Pseudomonas putida
Molecular orbitals
Data structures
Costs and Cost Analysis

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Health, Toxicology and Mutagenesis

Cite this

Huang, Ded Shih ; Whang, Thou-Jen ; Cheng, Fei Chen ; Wu, Ya Ping ; Wang, Yi Ting ; Luo, Wen I. ; Wang, Yane Shih. / Toxicity assessment of mono-substituted benzenes and phenols using a Pseudomonas initial oxygen uptake assay. In: Environmental Toxicology and Chemistry. 2005 ; Vol. 24, No. 2. pp. 253-260.
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Toxicity assessment of mono-substituted benzenes and phenols using a Pseudomonas initial oxygen uptake assay. / Huang, Ded Shih; Whang, Thou-Jen; Cheng, Fei Chen; Wu, Ya Ping; Wang, Yi Ting; Luo, Wen I.; Wang, Yane Shih.

In: Environmental Toxicology and Chemistry, Vol. 24, No. 2, 01.02.2005, p. 253-260.

Research output: Contribution to journalArticle

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