TR4 orphan nuclear receptor functions as an apoptosis modulator via regulation of Bcl-2 gene expression

Eungseok Kim, Wen Lung Ma, Din Lii Lin, Shigeki Inui, Yuh Ling Chen, Chawnshang Chang

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

While Bcl-2 plays an important role in cell apoptosis, its relationship to the orphan nuclear receptors remains unclear. Here we report that mouse embryonic fibroblast (MEF) cells prepared from TR4-deficient (TR4-/-) mice are more susceptible to UV-irradiation mediated apoptosis compared to TR4-Wildtype (TR4+/+) littermates. Substantial increasing TR4-/- MEF apoptosis to UV-irradiation was correlated to the down-regulation of Bcl-2 RNA and protein expression and collaterally increased caspase-3 activity. Furthermore, this TR4-induced Bcl-2 gene expression can be suppressed by co-transfection with TR4 coregulators, such as androgen receptor (AR) and receptor-interacting protein 140 (RIP140) in a dose-dependent manner. Together, our results demonstrate that TR4 might function as an apoptosis modulator through induction of Bcl-2 gene expression.

Original languageEnglish
Pages (from-to)323-328
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume361
Issue number2
DOIs
Publication statusPublished - 2007 Sep 21

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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