Transactivation of protein expression by rice HSP101 in planta and using Hsp101 as a selection marker for transformation

Ching-Chun Chang, Pin Sheng Huang, Huei Ru Lin, Chun Hsien Lu

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Plant HSP101 has dual activities, first, in conferring thermotolerance, and secondly, in serving as a translational activator. In this study, we introduced Oryza sativa Hsp101 (osHsp101) cDNA into tobacco by Agrobacterium-mediated transformation. Stable integration and expression of the transgene into the tobacco genome was demonstrated by Southern and Western blot analysis. Overexpression of osHSP101 had no noticeable effect on growth or development of the transgenic plants. Homozygous T2 transgenic plants with overexpressed osHSP101 survived heat treatment better than untransformed control plants. In addition, taking advantage of conferring basal thermotolerance by plant HSP101, we were able to demonstrate the feasibility of using osHsp101 as a selection marker and select the transformants under high temperature in tobacco leaf disc transformation mediated by Agrobacterium. Furthermore, transgenic tobacco plants with overexpressed osHSP101 were able to enhance luciferase expression up to 2.9-fold more than untransformed plants in the progeny of reciprocally crossed with omega-luciferase reporter lines.

Original languageEnglish
Pages (from-to)1098-1107
Number of pages10
JournalPlant and Cell Physiology
Volume48
Issue number8
DOIs
Publication statusPublished - 2007 Aug 1

Fingerprint

transcriptional activation
Plantae
Transcriptional Activation
Tobacco
Genetically Modified Plants
protein synthesis
rice
tobacco
Agrobacterium
Luciferases
luciferase
Proteins
heat tolerance
transgenic plants
Oryza sativa
Southern Blotting
Transgenes
Growth and Development
Complementary DNA
Hot Temperature

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

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title = "Transactivation of protein expression by rice HSP101 in planta and using Hsp101 as a selection marker for transformation",
abstract = "Plant HSP101 has dual activities, first, in conferring thermotolerance, and secondly, in serving as a translational activator. In this study, we introduced Oryza sativa Hsp101 (osHsp101) cDNA into tobacco by Agrobacterium-mediated transformation. Stable integration and expression of the transgene into the tobacco genome was demonstrated by Southern and Western blot analysis. Overexpression of osHSP101 had no noticeable effect on growth or development of the transgenic plants. Homozygous T2 transgenic plants with overexpressed osHSP101 survived heat treatment better than untransformed control plants. In addition, taking advantage of conferring basal thermotolerance by plant HSP101, we were able to demonstrate the feasibility of using osHsp101 as a selection marker and select the transformants under high temperature in tobacco leaf disc transformation mediated by Agrobacterium. Furthermore, transgenic tobacco plants with overexpressed osHSP101 were able to enhance luciferase expression up to 2.9-fold more than untransformed plants in the progeny of reciprocally crossed with omega-luciferase reporter lines.",
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Transactivation of protein expression by rice HSP101 in planta and using Hsp101 as a selection marker for transformation. / Chang, Ching-Chun; Huang, Pin Sheng; Lin, Huei Ru; Lu, Chun Hsien.

In: Plant and Cell Physiology, Vol. 48, No. 8, 01.08.2007, p. 1098-1107.

Research output: Contribution to journalArticle

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AB - Plant HSP101 has dual activities, first, in conferring thermotolerance, and secondly, in serving as a translational activator. In this study, we introduced Oryza sativa Hsp101 (osHsp101) cDNA into tobacco by Agrobacterium-mediated transformation. Stable integration and expression of the transgene into the tobacco genome was demonstrated by Southern and Western blot analysis. Overexpression of osHSP101 had no noticeable effect on growth or development of the transgenic plants. Homozygous T2 transgenic plants with overexpressed osHSP101 survived heat treatment better than untransformed control plants. In addition, taking advantage of conferring basal thermotolerance by plant HSP101, we were able to demonstrate the feasibility of using osHsp101 as a selection marker and select the transformants under high temperature in tobacco leaf disc transformation mediated by Agrobacterium. Furthermore, transgenic tobacco plants with overexpressed osHSP101 were able to enhance luciferase expression up to 2.9-fold more than untransformed plants in the progeny of reciprocally crossed with omega-luciferase reporter lines.

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