Abstract
Recent clinical study indicated that up-regulation of miR-146b was associated with poor overall survival of patients in esophageal squamous cell carcinoma. However, the underlying mechanism of miR-146b dysregulation remains to be explored. Here we report that miR-146b promotes cell proliferation and inhibits cell apoptosis in esophageal cancer cell lines. Mechanismly, two C/EBPβ binding motifs are located in the miR-146b promoter conserved region. Among the three isoforms of C/EBPβ, C/EBPβ LAP2 positively regulated miR-146b expression and increases miR-146b levels in a dose-dependent manner through transcription activation of miR-146b gene. Together, these results suggest a miR-146b regulatory mechanism involving C/EBPβ, which may contribute to the up-regulation of miR-146b in esophageal squamous cell carcinoma.
| Original language | English |
|---|---|
| Pages (from-to) | 267-271 |
| Number of pages | 5 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 446 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2014 Mar 28 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
Cite this
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Transcriptional regulation of miR-146b by C/EBPβ LAP2 in esophageal cancer cells. / Li, Junxia; Shan, Fabo; Xiong, Gang; Wang, Ju-Ming; Wang, Wen Lin; Xu, Xueqing; Bai, Yun.
In: Biochemical and Biophysical Research Communications, Vol. 446, No. 1, 28.03.2014, p. 267-271.Research output: Contribution to journal › Article
TY - JOUR
T1 - Transcriptional regulation of miR-146b by C/EBPβ LAP2 in esophageal cancer cells
AU - Li, Junxia
AU - Shan, Fabo
AU - Xiong, Gang
AU - Wang, Ju-Ming
AU - Wang, Wen Lin
AU - Xu, Xueqing
AU - Bai, Yun
PY - 2014/3/28
Y1 - 2014/3/28
N2 - Recent clinical study indicated that up-regulation of miR-146b was associated with poor overall survival of patients in esophageal squamous cell carcinoma. However, the underlying mechanism of miR-146b dysregulation remains to be explored. Here we report that miR-146b promotes cell proliferation and inhibits cell apoptosis in esophageal cancer cell lines. Mechanismly, two C/EBPβ binding motifs are located in the miR-146b promoter conserved region. Among the three isoforms of C/EBPβ, C/EBPβ LAP2 positively regulated miR-146b expression and increases miR-146b levels in a dose-dependent manner through transcription activation of miR-146b gene. Together, these results suggest a miR-146b regulatory mechanism involving C/EBPβ, which may contribute to the up-regulation of miR-146b in esophageal squamous cell carcinoma.
AB - Recent clinical study indicated that up-regulation of miR-146b was associated with poor overall survival of patients in esophageal squamous cell carcinoma. However, the underlying mechanism of miR-146b dysregulation remains to be explored. Here we report that miR-146b promotes cell proliferation and inhibits cell apoptosis in esophageal cancer cell lines. Mechanismly, two C/EBPβ binding motifs are located in the miR-146b promoter conserved region. Among the three isoforms of C/EBPβ, C/EBPβ LAP2 positively regulated miR-146b expression and increases miR-146b levels in a dose-dependent manner through transcription activation of miR-146b gene. Together, these results suggest a miR-146b regulatory mechanism involving C/EBPβ, which may contribute to the up-regulation of miR-146b in esophageal squamous cell carcinoma.
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UR - http://www.scopus.com/inward/citedby.url?scp=84897982441&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2014.02.096
DO - 10.1016/j.bbrc.2014.02.096
M3 - Article
VL - 446
SP - 267
EP - 271
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -