Transcriptional regulation of volatile terpenoid biosynthesis in phalaenopsis orchids

Yu Chen Chuang, Hong Hwa Chen

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

A synchronized floral development and monoterpenes biosynthesis as floral scents are assumed to attract pollinators in Phalaenopsis bellina, but their coordination regulation is still unclear. To unveil the developmental regulation of monoterpenes biosynthesis, we analyze the volatiles and transcriptomes of the scent and scentless orchids at various floral developmental stages. We identify differentially expressed structural genes and isolate the geranyl diphosphate gene PbGDPS, whose ectopic transient expression leads to monoterpene elevation in scentless orchids. The GDPS promoters from scent (designated PbGDPSp) and scentless (designated PaGDPSpA and PaGDPSpB) orchids are next isolated. The promoter activity of PbGDPSp and PaGDPSpA is much higher in the scent than in the scentless orchids. The nucleotide sequence of PaGDPSpB differs from the other two, and its transactivation is barely detectable in planta. We then isolate five transcription factors (TFs) with sequential elevation expression throughout the floral development in P. bellina, and transient assays indicate that they transactivate promoters of structural genes. Further ectopic transient expression of the five TFs in scentless orchids demonstrates that their roles in promoting monoterpene production. Our study reveals an elaborate control of monoterpenes biosynthesis involving sequential and coordinated expression of TFs and structural genes throughout the orchid floral development.

Original languageEnglish
Title of host publicationOrchid Biotechnology Iv
PublisherWorld Scientific Publishing Co. Pte Ltd
Pages327-357
Number of pages31
ISBN (Electronic)9789811217777
ISBN (Print)9789811217760
Publication statusPublished - 2021 Mar 23

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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