TY - JOUR
T1 - Transcriptome of Escherichia coli K1 bound to human brain microvascular endothelial cells
AU - Xie, Yi
AU - Parthasarathy, Geetha
AU - Di Cello, Francescopaolo
AU - Teng, Ching Hao
AU - Paul-Satyaseela, Maneesh
AU - Kim, Kwang Sik
N1 - Funding Information:
This work was supported by American Heart Association BGIA-0665442U (to Y.X.) and National Institutes of Health Grants RO1-NS 26310 and AI 71894 (to K.S.K.).
PY - 2008/1/4
Y1 - 2008/1/4
N2 - Escherichia coli K1 is the most common Gram-negative organism causing neonatal meningitis. Binding to human brain microvascular endothelial cells (HBMEC) is an essential step for E. coli K1 traversal of the blood-brain barrier. In this study, we examined expression profiles of E. coli K1 strain RS218 during its binding to HBMEC. Comparison of HBMEC-bound E. coli K1 with collagen-bound E. coli revealed more than one hundred genes whose expression patterns were significantly changed in HBMEC-bound E. coli K1, but not in collagen-bound E. coli K1. These genes are involved mainly in cell surface decorations, cellular function, and nitrogen metabolism. The roles of several representative genes including frdA, clpB, carA, and ompT in HBMEC binding were verified with their isogenic mutants, which exhibited significantly less HBMEC binding capability compared to that of the parent strain. This transcriptome analysis provided us with the first genomic-level view of E. coli and HBMEC interactions.
AB - Escherichia coli K1 is the most common Gram-negative organism causing neonatal meningitis. Binding to human brain microvascular endothelial cells (HBMEC) is an essential step for E. coli K1 traversal of the blood-brain barrier. In this study, we examined expression profiles of E. coli K1 strain RS218 during its binding to HBMEC. Comparison of HBMEC-bound E. coli K1 with collagen-bound E. coli revealed more than one hundred genes whose expression patterns were significantly changed in HBMEC-bound E. coli K1, but not in collagen-bound E. coli K1. These genes are involved mainly in cell surface decorations, cellular function, and nitrogen metabolism. The roles of several representative genes including frdA, clpB, carA, and ompT in HBMEC binding were verified with their isogenic mutants, which exhibited significantly less HBMEC binding capability compared to that of the parent strain. This transcriptome analysis provided us with the first genomic-level view of E. coli and HBMEC interactions.
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U2 - 10.1016/j.bbrc.2007.10.174
DO - 10.1016/j.bbrc.2007.10.174
M3 - Article
C2 - 17983591
AN - SCOPUS:36049016402
SN - 0006-291X
VL - 365
SP - 201
EP - 206
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -