Transcriptome of Escherichia coli K1 bound to human brain microvascular endothelial cells

Yi Xie, Geetha Parthasarathy, Francescopaolo Di Cello, Ching Hao Teng, Maneesh Paul-Satyaseela, Kwang Sik Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Escherichia coli K1 is the most common Gram-negative organism causing neonatal meningitis. Binding to human brain microvascular endothelial cells (HBMEC) is an essential step for E. coli K1 traversal of the blood-brain barrier. In this study, we examined expression profiles of E. coli K1 strain RS218 during its binding to HBMEC. Comparison of HBMEC-bound E. coli K1 with collagen-bound E. coli revealed more than one hundred genes whose expression patterns were significantly changed in HBMEC-bound E. coli K1, but not in collagen-bound E. coli K1. These genes are involved mainly in cell surface decorations, cellular function, and nitrogen metabolism. The roles of several representative genes including frdA, clpB, carA, and ompT in HBMEC binding were verified with their isogenic mutants, which exhibited significantly less HBMEC binding capability compared to that of the parent strain. This transcriptome analysis provided us with the first genomic-level view of E. coli and HBMEC interactions.

Original languageEnglish
Pages (from-to)201-206
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume365
Issue number1
DOIs
Publication statusPublished - 2008 Jan 4

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Endothelial cells
Transcriptome
Escherichia coli
Brain
Endothelial Cells
Collagen
Genes
Gene Expression Profiling
Blood-Brain Barrier
Meningitis
Metabolism
Gene expression
Cell Communication
Nitrogen
Gene Expression

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Xie, Yi ; Parthasarathy, Geetha ; Di Cello, Francescopaolo ; Teng, Ching Hao ; Paul-Satyaseela, Maneesh ; Kim, Kwang Sik. / Transcriptome of Escherichia coli K1 bound to human brain microvascular endothelial cells. In: Biochemical and Biophysical Research Communications. 2008 ; Vol. 365, No. 1. pp. 201-206.
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Transcriptome of Escherichia coli K1 bound to human brain microvascular endothelial cells. / Xie, Yi; Parthasarathy, Geetha; Di Cello, Francescopaolo; Teng, Ching Hao; Paul-Satyaseela, Maneesh; Kim, Kwang Sik.

In: Biochemical and Biophysical Research Communications, Vol. 365, No. 1, 04.01.2008, p. 201-206.

Research output: Contribution to journalArticle

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