TY - JOUR
T1 - Transient induction of apoptosis in serum-starved glioma cells by insulin and IGF-1
AU - Yang, Bei Chang
AU - Chang, Han Ming
AU - Wang, Yuh Sheng
AU - Chen, Rong Fu
AU - Lin, Shio Jean
N1 - Funding Information:
This research was supported by grants from the National Science Council, ROC, to B.C.Y. (NSC 83-0412-B006-056) and to S.J.L (NSC-83-0412-B006-061). The authors thank Drs. K.Y. Huang and L.Y. Chen for critical reading of the manuscript.
PY - 1996/11/8
Y1 - 1996/11/8
N2 - Insulin has a wide variety of biological effects. One of them is a mitogen-like activity whereby cell proliferation is stimulated. In this study we found a heretofore unreported insulin-elicited transient apoptosis of glioma cells. When serum-starved glioma cells were fed with a fresh regular medium, in the 6- to 12-h post-starvation period, the growth rate as determined by cell number was significantly suppressed by insulin, although cell cycle progression and DNA synthesis were actually accelerated. Increase in apoptosis in those growth-retarded cultures was demonstrable by Hoechst staining, detection of histone-associated DNA fragment, and in situ cell death detection. Apoptosis occurred among cells in all stages of cell cycle. After 24 h post-starvation, insulin increased the total cell number like a typical growth-promoting mitogen. In this regard, IGF-1, but not EGF nor TGF-β1, behaved like insulin.
AB - Insulin has a wide variety of biological effects. One of them is a mitogen-like activity whereby cell proliferation is stimulated. In this study we found a heretofore unreported insulin-elicited transient apoptosis of glioma cells. When serum-starved glioma cells were fed with a fresh regular medium, in the 6- to 12-h post-starvation period, the growth rate as determined by cell number was significantly suppressed by insulin, although cell cycle progression and DNA synthesis were actually accelerated. Increase in apoptosis in those growth-retarded cultures was demonstrable by Hoechst staining, detection of histone-associated DNA fragment, and in situ cell death detection. Apoptosis occurred among cells in all stages of cell cycle. After 24 h post-starvation, insulin increased the total cell number like a typical growth-promoting mitogen. In this regard, IGF-1, but not EGF nor TGF-β1, behaved like insulin.
UR - http://www.scopus.com/inward/record.url?scp=0030575857&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030575857&partnerID=8YFLogxK
U2 - 10.1016/S0167-4889(96)00079-1
DO - 10.1016/S0167-4889(96)00079-1
M3 - Article
C2 - 8972721
AN - SCOPUS:0030575857
SN - 0167-4889
VL - 1314
SP - 83
EP - 92
JO - Biochimica et Biophysica Acta - Molecular Cell Research
JF - Biochimica et Biophysica Acta - Molecular Cell Research
IS - 1-2
ER -