Transient induction of apoptosis in serum-starved glioma cells by insulin and IGF-1

Bei Chang Yang, Han Ming Chang, Yuh Sheng Wang, Rong Fu Chen, Shio Jean Lin

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)


Insulin has a wide variety of biological effects. One of them is a mitogen-like activity whereby cell proliferation is stimulated. In this study we found a heretofore unreported insulin-elicited transient apoptosis of glioma cells. When serum-starved glioma cells were fed with a fresh regular medium, in the 6- to 12-h post-starvation period, the growth rate as determined by cell number was significantly suppressed by insulin, although cell cycle progression and DNA synthesis were actually accelerated. Increase in apoptosis in those growth-retarded cultures was demonstrable by Hoechst staining, detection of histone-associated DNA fragment, and in situ cell death detection. Apoptosis occurred among cells in all stages of cell cycle. After 24 h post-starvation, insulin increased the total cell number like a typical growth-promoting mitogen. In this regard, IGF-1, but not EGF nor TGF-β1, behaved like insulin.

Original languageEnglish
Pages (from-to)83-92
Number of pages10
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Issue number1-2
Publication statusPublished - 1996 Nov 8

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Transient induction of apoptosis in serum-starved glioma cells by insulin and IGF-1'. Together they form a unique fingerprint.

Cite this