Transparent arrays of bilayer-nanomesh microelectrodes for simultaneous electrophysiology and two-photon imaging in the brain

Yi Qiang, Pietro Artoni, Kyung Jin Seo, Stanislav Culaclii, Victoria Hogan, Xuanyi Zhao, Yiding Zhong, Xun Han, Po Min Wang, Yi Kai Lo, Yueming Li, Henil A. Patel, Yifu Huang, Abhijeet Sambangi, Jung Soo V. Chu, Wentai Liu, Michela Fagiolini, Hui Fang

Research output: Contribution to journalArticlepeer-review

104 Citations (Scopus)

Abstract

Transparent microelectrode arrays have emerged as increasingly important tools for neuroscience by allowing simultaneous coupling of big and time-resolved electrophysiology data with optically measured, spatially and type resolved single neuron activity. Scaling down transparent electrodes to the length scale of a single neuron is challenging since conventional transparent conductors are limited by their capacitive electrode/electrolyte interface. In this study, we establish transparent microelectrode arrays with high performance, great biocompatibility, and comprehensive in vivo validations from a recently developed, bilayer-nanomesh material composite, where a metal layer and a low-impedance faradaic interfacial layer are stacked reliably together in a same transparent nanomesh pattern. Specifically, flexible arrays from 32 bilayer-nanomesh microelectrodes demonstrated near-unity yield with high uniformity, excellent biocompatibility, and great compatibility with state-of-the-art wireless recording and real-time artifact rejection system. The electrodes are highly scalable, with 130 kilohms at 1 kHz at 20 m in diameter, comparable to the performance of microelectrodes in nontransparent Michigan arrays. The highly transparent, bilayer-nanomesh microelectrode arrays allowed in vivo two-photon imaging of single neurons in layer 2/3 of the visual cortex of awake mice, along with high-fidelity, simultaneous electrical recordings of visual-evoked activity, both in the multi-unit activity band and at lower frequencies by measuring the visual-evoked potential in the time domain. Together, these advances reveal the great potential of transparent arrays from bilayer-nanomesh microelectrodes for a broad range of utility in neuroscience and medical practices.

Original languageEnglish
Article numbereaat0626
JournalScience Advances
Volume4
Issue number9
DOIs
Publication statusPublished - 2018

All Science Journal Classification (ASJC) codes

  • General

Fingerprint

Dive into the research topics of 'Transparent arrays of bilayer-nanomesh microelectrodes for simultaneous electrophysiology and two-photon imaging in the brain'. Together they form a unique fingerprint.

Cite this