TY - JOUR
T1 - Transplantation of porcine embryonic stem cells and their derived neuronal progenitors in a spinal cord injury rat model
AU - Yang, Jenn Rong
AU - Liao, Chia Hsin
AU - Pang, Cheng Yoong
AU - Huang, Lynn Ling Huei
AU - Chen, Yi Ling
AU - Shiue, Yow Ling
AU - Chen, Lih Ren
N1 - Funding Information:
This work was supported by grants 98AS-1.1.3-LI-L1 (2) from the Livestock Research Institute, Council of Agriculture and TCSP-01-02 by Buddhist Tzu-Chi General Hospital , Taiwan.
PY - 2013/2
Y1 - 2013/2
N2 - Background aims. The purpose of this study was to investigate therapeutic potential of green fluorescent protein expressing porcine embryonic stem (pES/GFP+) cells in A rat model of spinal cord injury (SCI). Methods. Undifferentiated pES/GFP+ cells and their neuronal differentiation derivatives were transplanted into the contused spinal cord of the Long Evans rat, and in situ development of the cells was determined by using a live animal fluorescence optical imaging system every 15 days. After pES/GFP+ cell transplantation, the behavior functional recovery of the SCI rats was assessed with the Basso, Beattie, and Bresnahan Locomotor Rating Scale (BBB scale), and the growth and differentiation of the grafted pES/GFP+ cells in the SCI rats were analyzed by immunohistochemical staining. Results. The relative green fluorescent protein expression level was decreased for 3 months after transplantation. The pES/GFP+-derived cells positively stained with neural specific antibodies of anti-NFL, anti-MBP, anti-SYP and anti-Tuj 1 were detected at the transplanted position. The SCI rats grafted with the D18 neuronal progenitors showed a significant functional recovery of hindlimbs and exhibited the highest BBB scale score of 15.20 ±1.43 at week 24. The SCI rats treated with pES/GFP+-derived neural progenitors demonstrated a better functional recovery. Conclusions. Transplantation of porcine embryonic stem (pES)-derived D18 neuronal progenitors has treatment potential for SCI, and functional behavior improvement of grafted pES-derived cells in SCI model rats suggests the potential for further application of pES cells in the study of replacement medicine and functionally degenerative pathologies.
AB - Background aims. The purpose of this study was to investigate therapeutic potential of green fluorescent protein expressing porcine embryonic stem (pES/GFP+) cells in A rat model of spinal cord injury (SCI). Methods. Undifferentiated pES/GFP+ cells and their neuronal differentiation derivatives were transplanted into the contused spinal cord of the Long Evans rat, and in situ development of the cells was determined by using a live animal fluorescence optical imaging system every 15 days. After pES/GFP+ cell transplantation, the behavior functional recovery of the SCI rats was assessed with the Basso, Beattie, and Bresnahan Locomotor Rating Scale (BBB scale), and the growth and differentiation of the grafted pES/GFP+ cells in the SCI rats were analyzed by immunohistochemical staining. Results. The relative green fluorescent protein expression level was decreased for 3 months after transplantation. The pES/GFP+-derived cells positively stained with neural specific antibodies of anti-NFL, anti-MBP, anti-SYP and anti-Tuj 1 were detected at the transplanted position. The SCI rats grafted with the D18 neuronal progenitors showed a significant functional recovery of hindlimbs and exhibited the highest BBB scale score of 15.20 ±1.43 at week 24. The SCI rats treated with pES/GFP+-derived neural progenitors demonstrated a better functional recovery. Conclusions. Transplantation of porcine embryonic stem (pES)-derived D18 neuronal progenitors has treatment potential for SCI, and functional behavior improvement of grafted pES-derived cells in SCI model rats suggests the potential for further application of pES cells in the study of replacement medicine and functionally degenerative pathologies.
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U2 - 10.1016/j.jcyt.2012.09.001
DO - 10.1016/j.jcyt.2012.09.001
M3 - Article
C2 - 23245953
AN - SCOPUS:84875442169
VL - 15
SP - 201
EP - 208
JO - Cytotherapy
JF - Cytotherapy
SN - 1465-3249
IS - 2
ER -
