True color blood flow imaging using a high-speed laser photography system

Chien Sheng Liu, Cheng Hsien Lin, Yung Nien Sun, Chung Liang Ho, Chung Chi Hsu

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3 Citations (Scopus)


Physiological changes in the retinal vasculature are commonly indicative of such disorders as diabetic retinopathy, glaucoma, and age-related macular degeneration. Thus, various methods have been developed for noninvasive clinical evaluation of ocular hemodynamics. However, to the best of our knowledge, current ophthalmic instruments do not provide a true color blood flow imaging capability. Accordingly, we propose a new method for the true color imaging of blood flow using a high-speed pulsed laser photography system. In the proposed approach, monochromatic images of the blood flow are acquired using a system of three cameras and three color lasers (red, green, and blue). A highquality true color image of the blood flow is obtained by assembling the monochromatic images by means of image realignment and color calibration processes. The effectiveness of the proposed approach is demonstrated by imaging the flow of mouse blood within a microfluidic channel device. The experimental results confirm the proposed system provides a high-quality true color blood flow imaging capability, and therefore has potential for noninvasive clinical evaluation of ocular hemodynamics.

Original languageEnglish
Article number103201
JournalOptical Engineering
Issue number10
Publication statusPublished - 2012 Oct 1


All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics
  • Engineering(all)

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