TY - JOUR
T1 - True color blood flow imaging using a high-speed laser photography system
AU - Liu, Chien Sheng
AU - Lin, Cheng Hsien
AU - Sun, Yung Nien
AU - Ho, Chung Liang
AU - Hsu, Chung Chi
N1 - Funding Information:
The authors gratefully acknowledge the financial support provided to this study by the National Science Council of Taiwan under Grant No. NSC 100-2218-E-194-008 and the Ministry of Economic Affairs of Taiwan under Grant No. 8301XS3E10.
PY - 2012/10
Y1 - 2012/10
N2 - Physiological changes in the retinal vasculature are commonly indicative of such disorders as diabetic retinopathy, glaucoma, and age-related macular degeneration. Thus, various methods have been developed for noninvasive clinical evaluation of ocular hemodynamics. However, to the best of our knowledge, current ophthalmic instruments do not provide a true color blood flow imaging capability. Accordingly, we propose a new method for the true color imaging of blood flow using a high-speed pulsed laser photography system. In the proposed approach, monochromatic images of the blood flow are acquired using a system of three cameras and three color lasers (red, green, and blue). A highquality true color image of the blood flow is obtained by assembling the monochromatic images by means of image realignment and color calibration processes. The effectiveness of the proposed approach is demonstrated by imaging the flow of mouse blood within a microfluidic channel device. The experimental results confirm the proposed system provides a high-quality true color blood flow imaging capability, and therefore has potential for noninvasive clinical evaluation of ocular hemodynamics.
AB - Physiological changes in the retinal vasculature are commonly indicative of such disorders as diabetic retinopathy, glaucoma, and age-related macular degeneration. Thus, various methods have been developed for noninvasive clinical evaluation of ocular hemodynamics. However, to the best of our knowledge, current ophthalmic instruments do not provide a true color blood flow imaging capability. Accordingly, we propose a new method for the true color imaging of blood flow using a high-speed pulsed laser photography system. In the proposed approach, monochromatic images of the blood flow are acquired using a system of three cameras and three color lasers (red, green, and blue). A highquality true color image of the blood flow is obtained by assembling the monochromatic images by means of image realignment and color calibration processes. The effectiveness of the proposed approach is demonstrated by imaging the flow of mouse blood within a microfluidic channel device. The experimental results confirm the proposed system provides a high-quality true color blood flow imaging capability, and therefore has potential for noninvasive clinical evaluation of ocular hemodynamics.
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U2 - 10.1117/1.OE.51.10.103201
DO - 10.1117/1.OE.51.10.103201
M3 - Article
AN - SCOPUS:84891799806
SN - 0091-3286
VL - 51
JO - Optical Engineering
JF - Optical Engineering
IS - 10
M1 - 103201
ER -
