Tumor targeting and MR imaging with lipophilic cyanine-mediated near-infrared responsive porous Gd silicate nanoparticles

Chen Sheng Yeh, Chia Hao Su, Wen Yueh Ho, Chih Chia Huang, Jui Cheng Chang, Yi Hsin Chien, Shu Ting Hung, Min Chiau Liau, Hsin Yi Ho

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

We synthesize a NIR MHI-148 dye, a lipophilic heptamethine cyanine, with capability in tumor-targeting property to accumulate in the mitochondria of tumor. In the context of MHI-148 dye, we demonstrate effective tumor targeting and NIR fluorescence invitro and invivo for MHI-148 as compared to ICG. A series of porous Gd silicates related nanoparticles, i.e. Gd silicate, Gd silicate@mSiO2 (mSiO2: mesoporous silica shell), and Gd3+-chelated Gd silicate@mSiO2 (Gd3+-DOTA chelated on the mSiO2) are fabricated to demonstrate their magnetic resonance (MR) contrast imaging effects. Those Gd silicates related nanoparticles exhibit dual MR effect, expressing T1-brightened and T2-darkened effects, in lower magnetic field. In high magnetic field, an abnormal enhanced transverse relaxivity (r2) appears, showing an effective T2-lowering effect, possibly due to concentrated Gd amount and porous architecture. The r2 value increases 4-5 times as the field strength increased from 3T to 7T. The Gd3+-chelated Gd silicate@mSiO2 has given large r2 (T2-lowering effect) up to 343.8s-1mm-1, which is even larger than the reported magnetic Fe3O4 nanoparticles measured at the same field. Using a 9.4T animal micro MRI system we have seen effectively darken in signal for those porous Gd silicates related NPs, while no such phenomenon appears in commercial Gd-DOTA agent. The MHI-148 is then conjugated on the porous Gd silicate@mSiO2 nanoparticles for a new paradigm with three functionalities for invivo tumor targeting, near-infrared fluorescent and MR imaging by means of only using MHI-148 dye.

Original languageEnglish
Pages (from-to)5677-5688
Number of pages12
JournalBiomaterials
Volume34
Issue number22
DOIs
Publication statusPublished - 2013 Jul

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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