Tumour necrosis factor-α causes an increase in blood-brain barrier permeability during sepsis

N. Tsao, Hui-Ping Hsu, C. M. Wu, Ching-Chuan Liu, H. Y. Lei

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

Blood-brain barrier (BBB) permeability during sepsis with Escherichia coli or Streptococcus pneumoniae was examined in a mouse model and measured by a circulating β-galactosidase tracer. The leakage of brain microvascular vessels during sepsis was confirmed by transmission electron microscopic examination of brain tissues stained with horseradish peroxidase. The increase of BBB permeability induced by E. coli and S. pneumoniae, which was maximal at 3 h and 12 h after injection, respectively, was transient because of rapid clearance of the bacteria from the blood. Tumour necrosis factor-α (TNF-α) was stained on microvascular vessels of the brain during sepsis and intravenous injection of recombinant TNF-α also increased the BBB permeability. The increase in BBB permeability induced by either E. coli or S. pneumoniae could be inhibited by anti-TNF-α antibody. It was concluded that circulating TNF-α generated during sepsis induced the increase in BBB permeability.

Original languageEnglish
Pages (from-to)812-821
Number of pages10
JournalJournal of Medical Microbiology
Volume50
Issue number9
DOIs
Publication statusPublished - 2001 Jan 1

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Blood-Brain Barrier
Permeability
Sepsis
Tumor Necrosis Factor-alpha
Streptococcus pneumoniae
Escherichia coli
Brain
Galactosidases
Horseradish Peroxidase
Intravenous Injections
Electrons
Bacteria
Injections
Antibodies

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

Cite this

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abstract = "Blood-brain barrier (BBB) permeability during sepsis with Escherichia coli or Streptococcus pneumoniae was examined in a mouse model and measured by a circulating β-galactosidase tracer. The leakage of brain microvascular vessels during sepsis was confirmed by transmission electron microscopic examination of brain tissues stained with horseradish peroxidase. The increase of BBB permeability induced by E. coli and S. pneumoniae, which was maximal at 3 h and 12 h after injection, respectively, was transient because of rapid clearance of the bacteria from the blood. Tumour necrosis factor-α (TNF-α) was stained on microvascular vessels of the brain during sepsis and intravenous injection of recombinant TNF-α also increased the BBB permeability. The increase in BBB permeability induced by either E. coli or S. pneumoniae could be inhibited by anti-TNF-α antibody. It was concluded that circulating TNF-α generated during sepsis induced the increase in BBB permeability.",
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Tumour necrosis factor-α causes an increase in blood-brain barrier permeability during sepsis. / Tsao, N.; Hsu, Hui-Ping; Wu, C. M.; Liu, Ching-Chuan; Lei, H. Y.

In: Journal of Medical Microbiology, Vol. 50, No. 9, 01.01.2001, p. 812-821.

Research output: Contribution to journalArticle

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T1 - Tumour necrosis factor-α causes an increase in blood-brain barrier permeability during sepsis

AU - Tsao, N.

AU - Hsu, Hui-Ping

AU - Wu, C. M.

AU - Liu, Ching-Chuan

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AB - Blood-brain barrier (BBB) permeability during sepsis with Escherichia coli or Streptococcus pneumoniae was examined in a mouse model and measured by a circulating β-galactosidase tracer. The leakage of brain microvascular vessels during sepsis was confirmed by transmission electron microscopic examination of brain tissues stained with horseradish peroxidase. The increase of BBB permeability induced by E. coli and S. pneumoniae, which was maximal at 3 h and 12 h after injection, respectively, was transient because of rapid clearance of the bacteria from the blood. Tumour necrosis factor-α (TNF-α) was stained on microvascular vessels of the brain during sepsis and intravenous injection of recombinant TNF-α also increased the BBB permeability. The increase in BBB permeability induced by either E. coli or S. pneumoniae could be inhibited by anti-TNF-α antibody. It was concluded that circulating TNF-α generated during sepsis induced the increase in BBB permeability.

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