Tunable magnetic alginate microbeads by using a spotting-based alginate microbead generator and Its applications for immunoassay-based diagnosis

Ruo Chi Hsu, Ming Yang Lin, Kang Yi Lien, Lein Yu Hung, Fong Yu Cheng, Chih-Chia Huang, Chen-Sheng Yeh, Huan Yao Lei, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This study presents a new method for generating tunable and uniform magnetic alginate microbeads, which can be widely employed in the field of the bead-based bioassays such as sample pretreatment or immunoassays. The proposed microfluidic chip comprises pneumatically-driven vibrators to continuously spot alginate microdroplets in a thin oil layer. Continuously, the temporarily formed alginate microdroplets sank into a CaCl2 solution to become gelled microbeads. Besides, 8 microchannels with radiation-shape arrangement were designed to equally distribute the alginate/magnetic-nanoparticles suspension supplied by only one syringe pump. In addition the sandwiched fluorescent immunoassay (FIA) for the detection of dengue virus can also be carried out by utilizing the magnetic alginate microbeads. Therefore, the tunable and uniform magnetic alginate microbeads can be successfully generated and demonstrated for biological applications.

Original languageEnglish
Title of host publicationNEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
Pages117-120
Number of pages4
DOIs
Publication statusPublished - 2011 Oct 4
Event6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011 - Kaohsiung, Taiwan
Duration: 2011 Feb 202011 Feb 23

Publication series

NameNEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems

Other

Other6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011
CountryTaiwan
CityKaohsiung
Period11-02-2011-02-23

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

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