Tunable magnetic alginate microbeads by using a spotting-based alginate microbead generator and Its applications for immunoassay-based diagnosis

Ruo Chi Hsu; Ming Yang Lin; Kang Yi Lien; Lein Yu Hung; Fong Yu Cheng; Chih Chia Huang; Chen Sheng Yeh; Huan Yao Lei; Gwo Bin Lee

doi:10.1109/NEMS.2011.6017309

Tunable magnetic alginate microbeads by using a spotting-based alginate microbead generator and Its applications for immunoassay-based diagnosis

Ruo Chi Hsu
, Ming Yang Lin
, Kang Yi Lien
, Lein Yu Hung
, Fong Yu Cheng
, Chih Chia Huang
, Chen Sheng Yeh
, Huan Yao Lei
, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

This study presents a new method for generating tunable and uniform magnetic alginate microbeads, which can be widely employed in the field of the bead-based bioassays such as sample pretreatment or immunoassays. The proposed microfluidic chip comprises pneumatically-driven vibrators to continuously spot alginate microdroplets in a thin oil layer. Continuously, the temporarily formed alginate microdroplets sank into a CaCl₂ solution to become gelled microbeads. Besides, 8 microchannels with radiation-shape arrangement were designed to equally distribute the alginate/magnetic-nanoparticles suspension supplied by only one syringe pump. In addition the sandwiched fluorescent immunoassay (FIA) for the detection of dengue virus can also be carried out by utilizing the magnetic alginate microbeads. Therefore, the tunable and uniform magnetic alginate microbeads can be successfully generated and demonstrated for biological applications.

Original language	English
Title of host publication	NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
Pages	117-120
Number of pages	4
DOIs	https://doi.org/10.1109/NEMS.2011.6017309
Publication status	Published - 2011
Event	6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011 - Kaohsiung, Taiwan Duration: 2011 Feb 20 → 2011 Feb 23

Publication series

Name	NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems

Other

Other	6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011
Country/Territory	Taiwan
City	Kaohsiung
Period	11-02-20 → 11-02-23

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Atomic and Molecular Physics, and Optics

Access to Document

10.1109/NEMS.2011.6017309

Cite this

Hsu, R. C., Lin, M. Y., Lien, K. Y., Hung, L. Y., Cheng, F. Y., Huang, C. C., Yeh, C. S., Lei, H. Y., & Lee, G. B. (2011). Tunable magnetic alginate microbeads by using a spotting-based alginate microbead generator and Its applications for immunoassay-based diagnosis. In NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (pp. 117-120). Article 6017309 (NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems). https://doi.org/10.1109/NEMS.2011.6017309

Hsu, Ruo Chi ; Lin, Ming Yang ; Lien, Kang Yi et al. / Tunable magnetic alginate microbeads by using a spotting-based alginate microbead generator and Its applications for immunoassay-based diagnosis. NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems. 2011. pp. 117-120 (NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems).

@inproceedings{fc397d0d8929425aa5d870dff3a8cffb,

title = "Tunable magnetic alginate microbeads by using a spotting-based alginate microbead generator and Its applications for immunoassay-based diagnosis",

abstract = "This study presents a new method for generating tunable and uniform magnetic alginate microbeads, which can be widely employed in the field of the bead-based bioassays such as sample pretreatment or immunoassays. The proposed microfluidic chip comprises pneumatically-driven vibrators to continuously spot alginate microdroplets in a thin oil layer. Continuously, the temporarily formed alginate microdroplets sank into a CaCl2 solution to become gelled microbeads. Besides, 8 microchannels with radiation-shape arrangement were designed to equally distribute the alginate/magnetic-nanoparticles suspension supplied by only one syringe pump. In addition the sandwiched fluorescent immunoassay (FIA) for the detection of dengue virus can also be carried out by utilizing the magnetic alginate microbeads. Therefore, the tunable and uniform magnetic alginate microbeads can be successfully generated and demonstrated for biological applications.",

author = "Hsu, \{Ruo Chi\} and Lin, \{Ming Yang\} and Lien, \{Kang Yi\} and Hung, \{Lein Yu\} and Cheng, \{Fong Yu\} and Huang, \{Chih Chia\} and Yeh, \{Chen Sheng\} and Lei, \{Huan Yao\} and Lee, \{Gwo Bin\}",

year = "2011",

doi = "10.1109/NEMS.2011.6017309",

language = "English",

isbn = "9781612847757",

series = "NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems",

pages = "117--120",

booktitle = "NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems",

note = "6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011 ; Conference date: 20-02-2011 Through 23-02-2011",

}

Hsu, RC, Lin, MY, Lien, KY, Hung, LY, Cheng, FY, Huang, CC , Yeh, CS, Lei, HY & Lee, GB 2011, Tunable magnetic alginate microbeads by using a spotting-based alginate microbead generator and Its applications for immunoassay-based diagnosis. in NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems., 6017309, NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, pp. 117-120, 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011, Kaohsiung, Taiwan, 11-02-20. https://doi.org/10.1109/NEMS.2011.6017309

Tunable magnetic alginate microbeads by using a spotting-based alginate microbead generator and Its applications for immunoassay-based diagnosis. / Hsu, Ruo Chi; Lin, Ming Yang; Lien, Kang Yi et al.
NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems. 2011. p. 117-120 6017309 (NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

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AU - Hsu, Ruo Chi

AU - Lin, Ming Yang

AU - Lien, Kang Yi

AU - Hung, Lein Yu

AU - Cheng, Fong Yu

AU - Huang, Chih Chia

AU - Yeh, Chen Sheng

AU - Lei, Huan Yao

AU - Lee, Gwo Bin

PY - 2011

Y1 - 2011

N2 - This study presents a new method for generating tunable and uniform magnetic alginate microbeads, which can be widely employed in the field of the bead-based bioassays such as sample pretreatment or immunoassays. The proposed microfluidic chip comprises pneumatically-driven vibrators to continuously spot alginate microdroplets in a thin oil layer. Continuously, the temporarily formed alginate microdroplets sank into a CaCl2 solution to become gelled microbeads. Besides, 8 microchannels with radiation-shape arrangement were designed to equally distribute the alginate/magnetic-nanoparticles suspension supplied by only one syringe pump. In addition the sandwiched fluorescent immunoassay (FIA) for the detection of dengue virus can also be carried out by utilizing the magnetic alginate microbeads. Therefore, the tunable and uniform magnetic alginate microbeads can be successfully generated and demonstrated for biological applications.

AB - This study presents a new method for generating tunable and uniform magnetic alginate microbeads, which can be widely employed in the field of the bead-based bioassays such as sample pretreatment or immunoassays. The proposed microfluidic chip comprises pneumatically-driven vibrators to continuously spot alginate microdroplets in a thin oil layer. Continuously, the temporarily formed alginate microdroplets sank into a CaCl2 solution to become gelled microbeads. Besides, 8 microchannels with radiation-shape arrangement were designed to equally distribute the alginate/magnetic-nanoparticles suspension supplied by only one syringe pump. In addition the sandwiched fluorescent immunoassay (FIA) for the detection of dengue virus can also be carried out by utilizing the magnetic alginate microbeads. Therefore, the tunable and uniform magnetic alginate microbeads can be successfully generated and demonstrated for biological applications.

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T3 - NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems

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BT - NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems

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Y2 - 20 February 2011 through 23 February 2011

ER -

Hsu RC, Lin MY, Lien KY, Hung LY, Cheng FY, Huang CC et al. Tunable magnetic alginate microbeads by using a spotting-based alginate microbead generator and Its applications for immunoassay-based diagnosis. In NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems. 2011. p. 117-120. 6017309. (NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems). doi: 10.1109/NEMS.2011.6017309

Tunable magnetic alginate microbeads by using a spotting-based alginate microbead generator and Its applications for immunoassay-based diagnosis

Abstract

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