TY - JOUR
T1 - Turn on the Mtr pathway genes under placi promoter in shewanella oneidensis MR-1
AU - Ng, I. Son
AU - Guo, Yanlan
AU - Zhou, Yunli
AU - Wu, Jhe Wei
AU - Tan, Shih I.
AU - Yi, Ying Chen
N1 - Funding Information:
The authors are grateful for the financial support received for this project from the Ministry of Science and Technology (MOST 105-2221-E-006-225-MY3 and MOST 105-2621-M-006-012-MY3) in Taiwan. We also sincerely appreciate the academic connection between Xiamen University (China) and National Cheng Kung University (Taiwan).
Funding Information:
The authors are grateful for the financial support received for this project from the Ministry of Science and Technology (MOST 105‑2221‑E‑006‑225‑MY3 and MOST 105‑2621‑M‑006‑012‑MY3) in Taiwan. We also sincerely appreciate the academic connection between Xiamen University (China) and National Cheng Kung University (Taiwan).
Publisher Copyright:
© The Author(s) 2018.
PY - 2018
Y1 - 2018
N2 - Background: Shewanella genus is famous for applications like electron transfer in microbe fuel cells and bioremediation of heavy metals through the Mtr pathway. A potential way to enhance the electron genesis ability of Shewanella is to express exogenous mtr genes via recombinant DNA technology. Thus, to design and develop expression vectors capable of replicating in Shewanella and enhance the genetic toolbox of the same is important. Result: In this study, a plasmid construct with a replication origin, repB, and pLacI promoter is reported for the first time to drive the expression of green fluorescent protein in S. oneidensis MR-1. Based on the same vector, the Mtr pathway genes mtrA, mtrC, and mtrCAB were also successfully expressed in MR-1. The recombinant strains had higher ferric reductase activity compared to the wild type. The highest enzymatic activity of 508.33 U/L in genetic Shewanella with mtrC gene is obtained, which is 1.53-fold higher than that of wild strain. The plasmids were stable up to 90 generations. Conclusion: We have demonstrated an expression system based on pLacI promoter and repB ori in Shewanella. Consequently, the combination of repB and pLacI will have great potential in Shewanella to turn on expression of different genes constitutively.
AB - Background: Shewanella genus is famous for applications like electron transfer in microbe fuel cells and bioremediation of heavy metals through the Mtr pathway. A potential way to enhance the electron genesis ability of Shewanella is to express exogenous mtr genes via recombinant DNA technology. Thus, to design and develop expression vectors capable of replicating in Shewanella and enhance the genetic toolbox of the same is important. Result: In this study, a plasmid construct with a replication origin, repB, and pLacI promoter is reported for the first time to drive the expression of green fluorescent protein in S. oneidensis MR-1. Based on the same vector, the Mtr pathway genes mtrA, mtrC, and mtrCAB were also successfully expressed in MR-1. The recombinant strains had higher ferric reductase activity compared to the wild type. The highest enzymatic activity of 508.33 U/L in genetic Shewanella with mtrC gene is obtained, which is 1.53-fold higher than that of wild strain. The plasmids were stable up to 90 generations. Conclusion: We have demonstrated an expression system based on pLacI promoter and repB ori in Shewanella. Consequently, the combination of repB and pLacI will have great potential in Shewanella to turn on expression of different genes constitutively.
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U2 - 10.1186/s40643-018-0221-9
DO - 10.1186/s40643-018-0221-9
M3 - Article
AN - SCOPUS:85066034856
SN - 2197-4365
VL - 5
JO - Bioresources and Bioprocessing
JF - Bioresources and Bioprocessing
IS - 1
M1 - 35
ER -
