Turning of the receptor-binding domains opens up the murine leukaemia virus Env for membrane fusion

Shang Rung Wu, Mathilda Sjöberg, Michael Wallin, Birgitta Lindqvist, Maria Ekström, Hans Hebert, Philip J.B. Koeck, Henrik Garoff

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The activity of the membrane fusion protein Env of Moloney mouse leukaemia virus is controlled by isomerization of the disulphide that couples its transmembrane (TM) and surface (SU) subunits. We have arrested Env activation at a stage prior to isomerization by alkylating the active thiol in SU and compared the structure of isomerization-arrested Env with that of native Env. Env trimers of respective form were isolated from solubilized particles by sedimentation and their structures were reconstructed from electron microscopic images of both vitrified and negatively stained samples. We found that the protomeric unit of both trimers formed three protrusions, a top, middle and a lower one. The atomic structure of the receptor-binding domain of SU fitted into the upper protrusion. This was formed similar to a bent finger. Significantly, in native Env the tips of the fingers were directed against each other enclosing a cavity below, whereas they had turned outward in isomerization-arrested Env transforming the cavity into an open well. This might subsequently guide the fusion peptides in extended TM subunits into the target membrane.

Original languageEnglish
Pages (from-to)2799-2808
Number of pages10
JournalEMBO Journal
Volume27
Issue number20
DOIs
Publication statusPublished - 2008 Oct 22

Fingerprint

Murine Leukemia Viruses
Virus Internalization
Isomerization
Viruses
Fingers
Fusion reactions
Membrane Fusion Proteins
Moloney murine leukemia virus
Membranes
Sulfhydryl Compounds
Disulfides
Electrons
Peptides
Sedimentation
Chemical activation

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Wu, S. R., Sjöberg, M., Wallin, M., Lindqvist, B., Ekström, M., Hebert, H., ... Garoff, H. (2008). Turning of the receptor-binding domains opens up the murine leukaemia virus Env for membrane fusion. EMBO Journal, 27(20), 2799-2808. https://doi.org/10.1038/emboj.2008.187
Wu, Shang Rung ; Sjöberg, Mathilda ; Wallin, Michael ; Lindqvist, Birgitta ; Ekström, Maria ; Hebert, Hans ; Koeck, Philip J.B. ; Garoff, Henrik. / Turning of the receptor-binding domains opens up the murine leukaemia virus Env for membrane fusion. In: EMBO Journal. 2008 ; Vol. 27, No. 20. pp. 2799-2808.
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Wu, SR, Sjöberg, M, Wallin, M, Lindqvist, B, Ekström, M, Hebert, H, Koeck, PJB & Garoff, H 2008, 'Turning of the receptor-binding domains opens up the murine leukaemia virus Env for membrane fusion', EMBO Journal, vol. 27, no. 20, pp. 2799-2808. https://doi.org/10.1038/emboj.2008.187

Turning of the receptor-binding domains opens up the murine leukaemia virus Env for membrane fusion. / Wu, Shang Rung; Sjöberg, Mathilda; Wallin, Michael; Lindqvist, Birgitta; Ekström, Maria; Hebert, Hans; Koeck, Philip J.B.; Garoff, Henrik.

In: EMBO Journal, Vol. 27, No. 20, 22.10.2008, p. 2799-2808.

Research output: Contribution to journalArticle

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AU - Hebert, Hans

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AU - Garoff, Henrik

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