Turntable paper-based device to detect Escherichia coli

Yung Chih Wang, Yao Hung Tsai, Ching Fen Shen, Ming Yao He, Yi Chen Fu, Chen Yu Sang, Yi Tzu Lee, Chao Min Cheng

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Escherichia coli has been known to cause a variety of infectious diseases. The conventional enzyme-linked immunosorbent assay (ELISA) is a well-known method widely used to diagnose a variety of infectious diseases. This method is expensive and requires considerable time and effort to conduct and complete multiple integral steps. We previously proposed the use of paper-based ELISA to rapidly detect the presence of E. coli. This approach has demonstrated utility for point-of-care (POC) urinary tract infection diagnoses. Paper-based ELISA, while advantageous, still requires the execution of several procedural steps. Here, we discuss the design and experimental implementation of a turntable paper-based device to simplify the paper-based ELISA protocols for the detection of E. coli. In this process, antibodies or reagents are preloaded onto zones of a paper-based device and allowed to dry before use. We successfully used this device to detect E. coli with a detection limit of 105 colony-forming units (colony-forming unit [CFU])/mL.

Original languageEnglish
Article number194
JournalMicromachines
Volume12
Issue number2
DOIs
Publication statusPublished - 2021 Feb

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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