Two-channel autofluorescence analysis for oral cancer

Tze Ta Huang, Ken Chung Chen, Tung Yiu Wong, Chih Yang Chen, Wang Ch Chen, Yi Chun Chen, Ming Hsuan Chang, Dong Yuan Wu, Teng Yi Huang, Shoko Nioka, Pau Choo Chung, Jehn Shyun Huang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We created a two-channel autofluorescence test to detect oral cancer. The wavelengths 375 and 460 nm, with filters of 479 and 525 nm, were designed to excite and detect reduced-form nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) autofluorescence. Patients with oral cancer or with precancerous lesions, and a control group with healthy oral mucosae, were enrolled. The lesion in the autofluorescent image was the region of interest. The average intensity and heterogeneity of the NADH and FAD were calculated. The redox ratio [(NADH)/(NADH + FAD)] was also computed. A quadratic discriminant analysis (QDA) was used to compute boundaries based on sensitivity and specificity. We analyzed 49 oral cancer lesions, 34 precancerous lesions, and 77 healthy oral mucosae. A boundary (sensitivity: 0.974 and specificity: 0.898) between the oral cancer lesions and healthy oral mucosae was validated. Oral cancer and precancerous lesions were also differentiated from healthy oral mucosae (sensitivity: 0.919 and specificity: 0.755). The two-channel autofluorescence detection device and analyses of the intensity and heterogeneity of NADH, and of FAD, and the redox ratio combined with a QDA classifier can differentiate oral cancer and precancerous lesions from healthy oral mucosae.

Original languageEnglish
Article number051402
JournalJournal of Biomedical Optics
Volume24
Issue number5
DOIs
Publication statusPublished - 2019 May 1

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Flavin-Adenine Dinucleotide
NAD
lesions
adenines
cancer
Discriminant analysis
sensitivity
nicotinamide
Classifiers
classifiers
Mucous Membrane
Wavelength
filters
wavelengths
Oxidation-Reduction

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

Cite this

Huang, Tze Ta ; Chen, Ken Chung ; Wong, Tung Yiu ; Chen, Chih Yang ; Chen, Wang Ch ; Chen, Yi Chun ; Chang, Ming Hsuan ; Wu, Dong Yuan ; Huang, Teng Yi ; Nioka, Shoko ; Chung, Pau Choo ; Huang, Jehn Shyun. / Two-channel autofluorescence analysis for oral cancer. In: Journal of Biomedical Optics. 2019 ; Vol. 24, No. 5.
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Huang, TT, Chen, KC, Wong, TY, Chen, CY, Chen, WC, Chen, YC, Chang, MH, Wu, DY, Huang, TY, Nioka, S, Chung, PC & Huang, JS 2019, 'Two-channel autofluorescence analysis for oral cancer', Journal of Biomedical Optics, vol. 24, no. 5, 051402. https://doi.org/10.1117/1.JBO.24.5.051402

Two-channel autofluorescence analysis for oral cancer. / Huang, Tze Ta; Chen, Ken Chung; Wong, Tung Yiu; Chen, Chih Yang; Chen, Wang Ch; Chen, Yi Chun; Chang, Ming Hsuan; Wu, Dong Yuan; Huang, Teng Yi; Nioka, Shoko; Chung, Pau Choo; Huang, Jehn Shyun.

In: Journal of Biomedical Optics, Vol. 24, No. 5, 051402, 01.05.2019.

Research output: Contribution to journalArticle

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AU - Chen, Wang Ch

AU - Chen, Yi Chun

AU - Chang, Ming Hsuan

AU - Wu, Dong Yuan

AU - Huang, Teng Yi

AU - Nioka, Shoko

AU - Chung, Pau Choo

AU - Huang, Jehn Shyun

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