Type I interferons protect mice against enterovirus 71 infection

Ming Liang Liu, Yi Ping Lee, Ya Fang Wang, Huan Yao Lei, Ching Chuan Liu, Shih Min Wang, Ih Jen Su, Jen Reng Wang, Trai Ming Yeh, Shun Hua Chen, Chun Keung Yu

Research output: Contribution to journalArticlepeer-review

109 Citations (Scopus)

Abstract

In this study, the contribution of type I interferons (IFNs) to protection against infection with enterovirus 71 (EV71) was investigated using a murine model where the virus was administrated to neonatal Institute of Cancer Research (ICR) mice by either the intraperitoneal (i.p.) or the oral route. In i.p. inoculated mice, post-infection treatment of dexamethasone (5 mg kg-1 at 2 or 3 days after infection) exacerbated clinical symptoms and increased the tissue viral titre. In contrast, polyriboinosinic: polyribocytidylic acid [poly(I:C); 10 or 100 μg per mouse at 12 h before infection], a potent IFN inducer, improved the survival rate and decreased the tissue viral titres after EV71 challenge, which correlated with an increase in serum IFN-α concentration, the percentage of dendritic cells, their expression of major histocompatibility complex class II molecule and IFN-α in spleen. Treatment with a neutralizing antibody for type I IFNs (104 neutralizing units per mouse, 6 h before and 12 h after infection) resulted in frequent deaths and higher tissue viral load in infected mice compared with control mice. In contrast, an early administration of recombinant mouse IFN-αA (104 U per mouse for 3 days starting at 0, 1 or 3 days after infection) protected the mice against EV71 infection. In vitro analysis of virus-induced death in three human cell lines showed that human type I IFNs exerted a direct protective effect on EV71. It was concluded that type I IFNs play an important role in controlling EV71 infection and replication.

Original languageEnglish
Pages (from-to)3263-3269
Number of pages7
JournalJournal of General Virology
Volume86
Issue number12
DOIs
Publication statusPublished - 2005 Dec

All Science Journal Classification (ASJC) codes

  • Virology

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