TY - JOUR
T1 - Ultra-low fouling and high antibody loading zwitterionic hydrogel coatings for sensing and detection in complex media
AU - Chou, Ying Nien
AU - Sun, Fang
AU - Hung, Hsiang Chieh
AU - Jain, Priyesh
AU - Sinclair, Andrew
AU - Zhang, Peng
AU - Bai, Tao
AU - Chang, Yung
AU - Wen, Ten Chin
AU - Yu, Qiuming
AU - Jiang, Shaoyi
N1 - Funding Information:
This work was supported by the National Science Foundation , United States ( CBET 1264470 ). Y.N.C., Y.C. and T.C.W. would like to acknowledge the PhD visiting student program and the projects of the Ministry of Science and Technology , Taiwan ( 102-2221-E-006-219-MY3 , 102-2221-E-033-009-MY3 and 103-2221-E-033-078-MY3 ) for their financial support.
Funding Information:
This work was supported by the National Science Foundation, United States (CBET 1264470). Y.N.C., Y.C. and T.C.W. would like to acknowledge the PhD visiting student program and the projects of the Ministry of Science and Technology, Taiwan (102-2221-E-006-219-MY3, 102-2221-E-033-009-MY3 and 103-2221-E-033-078-MY3) for their financial support.
Publisher Copyright:
© 2016 Acta Materialia Inc.
PY - 2016/8/1
Y1 - 2016/8/1
N2 - For surface-based diagnostic devices to achieve reliable biomarker detection in complex media such as blood, preventing nonspecific protein adsorption and incorporating high loading of biorecognition elements are paramount. In this work, a novel method to produce nonfouling zwitterionic hydrogel coatings was developed to achieve these goals. Poly(carboxybetaine acrylamide) (pCBAA) hydrogel thin films (CBHTFs) prepared with a carboxybetaine diacrylamide crosslinker (CBAAX) were coated on gold and silicon dioxide surfaces via a simple spin coating process. The thickness of CBHTFs could be precisely controlled between 15 and 150 nm by varying the crosslinker concentration, and the films demonstrated excellent long-term stability. Protein adsorption from undiluted human blood serum onto the CBHTFs was measured with surface plasmon resonance (SPR). Hydrogel thin films greater than 20 nm exhibited ultra-low fouling (<5 ng/cm2). In addition, the CBHTFs were capable of high antibody functionalization for specific biomarker detection without compromising their nonfouling performance. This strategy provides a facile method to modify SPR biosensor chips with an advanced nonfouling material, and can be potentially expanded to a variety of implantable medical devices and diagnostic biosensors. Statement of Significance In this work, we developed an approach to realize ultra-low fouling and high ligand loading with a highly-crosslinked, purely zwitterionic, carboxybetaine thin film hydrogel (CBHTF) coating platform. The CBHTF on a hydrophilic surface demonstrated long-term stability. By varying the crosslinker content in the spin-coated hydrogel solution, the thickness of CBHTFs could be precisely controlled. Optimized CBHTFs exhibited ultra-low nonspecific protein adsorption below 5 ng/cm2 measured by a surface plasmon resonance (SPR) sensor, and their 3D architecture allowed antibody loading to reach 693 ng/cm2. This strategy provides a facile method to modify SPR biosensor chips with an advanced nonfouling material, and can be potentially expanded to a variety of implantable medical devices and diagnostic biosensors.
AB - For surface-based diagnostic devices to achieve reliable biomarker detection in complex media such as blood, preventing nonspecific protein adsorption and incorporating high loading of biorecognition elements are paramount. In this work, a novel method to produce nonfouling zwitterionic hydrogel coatings was developed to achieve these goals. Poly(carboxybetaine acrylamide) (pCBAA) hydrogel thin films (CBHTFs) prepared with a carboxybetaine diacrylamide crosslinker (CBAAX) were coated on gold and silicon dioxide surfaces via a simple spin coating process. The thickness of CBHTFs could be precisely controlled between 15 and 150 nm by varying the crosslinker concentration, and the films demonstrated excellent long-term stability. Protein adsorption from undiluted human blood serum onto the CBHTFs was measured with surface plasmon resonance (SPR). Hydrogel thin films greater than 20 nm exhibited ultra-low fouling (<5 ng/cm2). In addition, the CBHTFs were capable of high antibody functionalization for specific biomarker detection without compromising their nonfouling performance. This strategy provides a facile method to modify SPR biosensor chips with an advanced nonfouling material, and can be potentially expanded to a variety of implantable medical devices and diagnostic biosensors. Statement of Significance In this work, we developed an approach to realize ultra-low fouling and high ligand loading with a highly-crosslinked, purely zwitterionic, carboxybetaine thin film hydrogel (CBHTF) coating platform. The CBHTF on a hydrophilic surface demonstrated long-term stability. By varying the crosslinker content in the spin-coated hydrogel solution, the thickness of CBHTFs could be precisely controlled. Optimized CBHTFs exhibited ultra-low nonspecific protein adsorption below 5 ng/cm2 measured by a surface plasmon resonance (SPR) sensor, and their 3D architecture allowed antibody loading to reach 693 ng/cm2. This strategy provides a facile method to modify SPR biosensor chips with an advanced nonfouling material, and can be potentially expanded to a variety of implantable medical devices and diagnostic biosensors.
UR - http://www.scopus.com/inward/record.url?scp=84963946834&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84963946834&partnerID=8YFLogxK
U2 - 10.1016/j.actbio.2016.04.023
DO - 10.1016/j.actbio.2016.04.023
M3 - Article
C2 - 27090589
AN - SCOPUS:84963946834
SN - 1742-7061
VL - 40
SP - 31
EP - 37
JO - Acta Biomaterialia
JF - Acta Biomaterialia
ER -