Ultra-stable and ultra-fast fabrication of microwells using laser ablation in plane PS for the formation of size-controlled hepatic tumor spheroids

Kuang Wei Wu, Zi Ting Huang, Chiao Yi Chiu, Ting Yuan Tu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This work presents an ultra-stable laser-ablated microwells for the formation of hepatic tumor spheroids. The microwells were fabricated by CO2 laser ablation on polystyrene (PS) surface for generating multicellular tumor spheroid, demonstrating a rapid and economical prototyping method. Laser commend pulses including frequency, number of pulses and duty cycle were investigated. We presented a reference to fabricate expected profiles of microwells easily by controlling frequency, number of pulses and duty cycle of laser system. Hepatocarcinoma tumor spheroids were grown in the microwells for long-term culture to test the feasibility of the current approach for generating multicellular tumor spheroids.

Original languageEnglish
Title of host publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PublisherChemical and Biological Microsystems Society
Pages1455-1457
Number of pages3
ISBN (Electronic)9781510897571
Publication statusPublished - 2018 Jan 1
Event22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan
Duration: 2018 Nov 112018 Nov 15

Publication series

Name22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Volume3

Conference

Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
CountryTaiwan
CityKaohsiung
Period18-11-1118-11-15

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Bioengineering
  • Chemical Engineering (miscellaneous)
  • Control and Systems Engineering

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  • Cite this

    Wu, K. W., Huang, Z. T., Chiu, C. Y., & Tu, T. Y. (2018). Ultra-stable and ultra-fast fabrication of microwells using laser ablation in plane PS for the formation of size-controlled hepatic tumor spheroids. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 (pp. 1455-1457). (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018; Vol. 3). Chemical and Biological Microsystems Society.