TY - GEN
T1 - Ultra-stable and ultra-fast fabrication of microwells using laser ablation in plane PS for the formation of size-controlled hepatic tumor spheroids
AU - Wu, Kuang Wei
AU - Huang, Zi Ting
AU - Chiu, Chiao Yi
AU - Tu, Ting Yuan
PY - 2018/1/1
Y1 - 2018/1/1
N2 - This work presents an ultra-stable laser-ablated microwells for the formation of hepatic tumor spheroids. The microwells were fabricated by CO2 laser ablation on polystyrene (PS) surface for generating multicellular tumor spheroid, demonstrating a rapid and economical prototyping method. Laser commend pulses including frequency, number of pulses and duty cycle were investigated. We presented a reference to fabricate expected profiles of microwells easily by controlling frequency, number of pulses and duty cycle of laser system. Hepatocarcinoma tumor spheroids were grown in the microwells for long-term culture to test the feasibility of the current approach for generating multicellular tumor spheroids.
AB - This work presents an ultra-stable laser-ablated microwells for the formation of hepatic tumor spheroids. The microwells were fabricated by CO2 laser ablation on polystyrene (PS) surface for generating multicellular tumor spheroid, demonstrating a rapid and economical prototyping method. Laser commend pulses including frequency, number of pulses and duty cycle were investigated. We presented a reference to fabricate expected profiles of microwells easily by controlling frequency, number of pulses and duty cycle of laser system. Hepatocarcinoma tumor spheroids were grown in the microwells for long-term culture to test the feasibility of the current approach for generating multicellular tumor spheroids.
UR - http://www.scopus.com/inward/record.url?scp=85079783080&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85079783080&partnerID=8YFLogxK
M3 - Conference contribution
T3 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
SP - 1455
EP - 1457
BT - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PB - Chemical and Biological Microsystems Society
T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Y2 - 11 November 2018 through 15 November 2018
ER -