TY - JOUR
T1 - Ultrasonic Transdermal Delivery System with Acid-Base Neutralization-Generated CO2 Microbubble Cavitation
AU - Ho, Yi Ju
AU - Hsu, Hui Ching
AU - Kang, Shih Tsung
AU - Fan, Ching Hsiang
AU - Chang, Chien Wen
AU - Yeh, Chih Kuang
N1 - Funding Information:
The authors gratefully acknowledge the support of the Ministry of Science and Technology, Taiwan under Grant No. MOST 108-2221-E-007-041-MY3, 108-2221-E-007-040-MY3, and 106-2218-E-007-022-MY3; National Tsing Hua University under Grant No. 108Q2717E1; Chang Gung Memorial Hospital (Linkou, Taiwan) under Grant No. 108Q2504E1.
Funding Information:
The authors gratefully acknowledge the support of the Ministry of Science and Technology, Taiwan under Grant No. MOST 108-2221-E-007-041-MY3 108-2221-E-007-040-MY3, and 106-2218-E-007-022-MY3; National Tsing Hua University under Grant No. 108Q2717E1; Chang Gung Memorial Hospital (Linkou, Taiwan) under Grant No. 108Q2504E1.
Publisher Copyright:
Copyright © 2020 American Chemical Society.
PY - 2020/4/20
Y1 - 2020/4/20
N2 - Transdermal delivery systems provide a convenient noninvasive approach for drug administration through the skin, and they have been widely developed for in-home health care. The stratum corneum of the skin surface limits drug penetration, but ultrasound (US)-stimulated microbubble (MB) cavitation can enhance skin permeability to promote transdermal drug penetration. However, the specific materials and complex fabrication of MBs influence the scope of application in transdermal delivery systems. Hence, we studied the mixture of citric acid and NaHCO3 agents to generate shell-free CO2-MBs by acid-base neutralization effect. The generation rate of CO2-MBs was 36.3 ± 10 MBs/s and the mean size was 110 ± 14 μm under US sonication (3.1 MHz, 0.5 W/cm2, 50% duty cycle, 1 min). The penetration of Evans blue and FITC-conjugated hyaluronic acid in rat abdominal skin by CO2-MB cavitation improved 2.4 ± 0.3 and 2.1 ± 0.1 fold, respectively. The penetration depth of Evans blue (27.1 ± 5.1 μm) reached the epidermal layer, providing the potential for inducing transcutaneous immunization. Therefore, we proposed a simple and self-operating ultrasonic transdermal delivery system with CO2-MB cavitation to improve drug penetration for in-home health care development.
AB - Transdermal delivery systems provide a convenient noninvasive approach for drug administration through the skin, and they have been widely developed for in-home health care. The stratum corneum of the skin surface limits drug penetration, but ultrasound (US)-stimulated microbubble (MB) cavitation can enhance skin permeability to promote transdermal drug penetration. However, the specific materials and complex fabrication of MBs influence the scope of application in transdermal delivery systems. Hence, we studied the mixture of citric acid and NaHCO3 agents to generate shell-free CO2-MBs by acid-base neutralization effect. The generation rate of CO2-MBs was 36.3 ± 10 MBs/s and the mean size was 110 ± 14 μm under US sonication (3.1 MHz, 0.5 W/cm2, 50% duty cycle, 1 min). The penetration of Evans blue and FITC-conjugated hyaluronic acid in rat abdominal skin by CO2-MB cavitation improved 2.4 ± 0.3 and 2.1 ± 0.1 fold, respectively. The penetration depth of Evans blue (27.1 ± 5.1 μm) reached the epidermal layer, providing the potential for inducing transcutaneous immunization. Therefore, we proposed a simple and self-operating ultrasonic transdermal delivery system with CO2-MB cavitation to improve drug penetration for in-home health care development.
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U2 - 10.1021/acsabm.9b01126
DO - 10.1021/acsabm.9b01126
M3 - Article
C2 - 35025319
AN - SCOPUS:85085100010
SN - 2576-6422
VL - 3
SP - 1968
EP - 1975
JO - ACS Applied Bio Materials
JF - ACS Applied Bio Materials
IS - 4
ER -