Ultrastructure of white spot syndrome virus development in primary lymphoid organ cell cultures

Chung Hsiung Wang, Hsi Nan Yang, Chih Yuan Tang, Chien Hsin Lu, Guang Hsiung Kou, Chu Fang Lo

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Primary cell cultures from the lymphoid organ of Penaeus monodon were used to investigate in vitro propagation and morphogenesis of white spot syndrome virus (WSSV). Double-strength Leibovitz's L15 supplemented with 20% fetal bovine serum, pH 7.5, with a final osmolarity of 530 ± 5 mOsm kg-1 was identified as the most suitable culture medium. In this medium, the lymphoid cells remained viable for more than 1 wk. Migrating cells were inoculated with WSSV, and the consequent cytopathic effects documented by light and electron microscopy. WSSV appears capable of following 2 alternative assembly sequences, one similar to the morphogenesis of the Oryctes rhinocerus virus and another which is more typical of baculoviral assembly. Possible relationships between WSSV, Oryctes virus, and baculoviruses are discussed.

Original languageEnglish
Pages (from-to)91-104
Number of pages14
JournalDiseases of Aquatic Organisms
Issue number2
Publication statusPublished - 2000 Jun 19

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

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