Unique clustering genes in the bacterial chromosome affecting the type-III secretion of enterohaemorrhagic Escherichia coli

I. Ting Lin, Yi Ming Chiou, Yen Chia Liang, Ching Nan Lin, Wei Sheng W. Sun, Shiaowen Li, Chuan Hsiung Chang, Wan Jr Syu, Jenn Wei Chen

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Bioinformatics analysis was used to search for unknown genes that might influence the phenotypic presentations of enterohaemorrhagic Escherichia coli (EHEC). By so doing and using the known genomic data from EHEC O157: H7 and K-12, it has been deduced that genes Z4863 to Z4866 of EHEC do not exist in K-12 strains. These four gene sequences have low degrees of homology (18-40% amino acid identities) to a set of genes in K-12, which have been known to encode fatty acid biosynthesis enzymes. We referred these four consecutive genes as a fasyn cluster and found that deletion of fasyn from EHEC resulted in a defective type-III secretion (T3S). This deletion apparently did not decrease the amounts of the T3S proteins ectopically expressed from plasmids. Examination of the corresponding mRNAs by real-time PCR revealed that the mRNAs readily decreased in the fasyn-deleted mutant and this suppressive effect on the mRNA levels appeared to spread across all lee operons. Complementation with fasyn reverted the T3S-deficient phenotype. Furthermore, this reversion was also seen when the mutant was supplemented with locus of enterocyte effacement activators (Ler or GrlA). Thus, these unique clustering genes located apart from locus of enterocyte effacement on the bacterial chromosome also play a role in affecting T3S of EHEC.

Original languageEnglish
Pages (from-to)1744-1754
Number of pages11
JournalMicrobiology (United Kingdom)
Volume162
Issue number10
DOIs
Publication statusPublished - 2016 Oct

Fingerprint

Bacterial Chromosomes
Enterohemorrhagic Escherichia coli
Cluster Analysis
Genes
Enterocytes
Messenger RNA
Escherichia coli O157
Operon
Computational Biology
Real-Time Polymerase Chain Reaction
Plasmids
Fatty Acids
Phenotype
Amino Acids
Enzymes

All Science Journal Classification (ASJC) codes

  • Microbiology

Cite this

Lin, I. Ting ; Chiou, Yi Ming ; Liang, Yen Chia ; Lin, Ching Nan ; Sun, Wei Sheng W. ; Li, Shiaowen ; Chang, Chuan Hsiung ; Syu, Wan Jr ; Chen, Jenn Wei. / Unique clustering genes in the bacterial chromosome affecting the type-III secretion of enterohaemorrhagic Escherichia coli. In: Microbiology (United Kingdom). 2016 ; Vol. 162, No. 10. pp. 1744-1754.
@article{c81e612be33043cfacedf686d1429e27,
title = "Unique clustering genes in the bacterial chromosome affecting the type-III secretion of enterohaemorrhagic Escherichia coli",
abstract = "Bioinformatics analysis was used to search for unknown genes that might influence the phenotypic presentations of enterohaemorrhagic Escherichia coli (EHEC). By so doing and using the known genomic data from EHEC O157: H7 and K-12, it has been deduced that genes Z4863 to Z4866 of EHEC do not exist in K-12 strains. These four gene sequences have low degrees of homology (18-40{\%} amino acid identities) to a set of genes in K-12, which have been known to encode fatty acid biosynthesis enzymes. We referred these four consecutive genes as a fasyn cluster and found that deletion of fasyn from EHEC resulted in a defective type-III secretion (T3S). This deletion apparently did not decrease the amounts of the T3S proteins ectopically expressed from plasmids. Examination of the corresponding mRNAs by real-time PCR revealed that the mRNAs readily decreased in the fasyn-deleted mutant and this suppressive effect on the mRNA levels appeared to spread across all lee operons. Complementation with fasyn reverted the T3S-deficient phenotype. Furthermore, this reversion was also seen when the mutant was supplemented with locus of enterocyte effacement activators (Ler or GrlA). Thus, these unique clustering genes located apart from locus of enterocyte effacement on the bacterial chromosome also play a role in affecting T3S of EHEC.",
author = "Lin, {I. Ting} and Chiou, {Yi Ming} and Liang, {Yen Chia} and Lin, {Ching Nan} and Sun, {Wei Sheng W.} and Shiaowen Li and Chang, {Chuan Hsiung} and Syu, {Wan Jr} and Chen, {Jenn Wei}",
year = "2016",
month = "10",
doi = "10.1099/mic.0.000348",
language = "English",
volume = "162",
pages = "1744--1754",
journal = "Microbiology",
issn = "1350-0872",
publisher = "Society for General Microbiology",
number = "10",

}

Unique clustering genes in the bacterial chromosome affecting the type-III secretion of enterohaemorrhagic Escherichia coli. / Lin, I. Ting; Chiou, Yi Ming; Liang, Yen Chia; Lin, Ching Nan; Sun, Wei Sheng W.; Li, Shiaowen; Chang, Chuan Hsiung; Syu, Wan Jr; Chen, Jenn Wei.

In: Microbiology (United Kingdom), Vol. 162, No. 10, 10.2016, p. 1744-1754.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Unique clustering genes in the bacterial chromosome affecting the type-III secretion of enterohaemorrhagic Escherichia coli

AU - Lin, I. Ting

AU - Chiou, Yi Ming

AU - Liang, Yen Chia

AU - Lin, Ching Nan

AU - Sun, Wei Sheng W.

AU - Li, Shiaowen

AU - Chang, Chuan Hsiung

AU - Syu, Wan Jr

AU - Chen, Jenn Wei

PY - 2016/10

Y1 - 2016/10

N2 - Bioinformatics analysis was used to search for unknown genes that might influence the phenotypic presentations of enterohaemorrhagic Escherichia coli (EHEC). By so doing and using the known genomic data from EHEC O157: H7 and K-12, it has been deduced that genes Z4863 to Z4866 of EHEC do not exist in K-12 strains. These four gene sequences have low degrees of homology (18-40% amino acid identities) to a set of genes in K-12, which have been known to encode fatty acid biosynthesis enzymes. We referred these four consecutive genes as a fasyn cluster and found that deletion of fasyn from EHEC resulted in a defective type-III secretion (T3S). This deletion apparently did not decrease the amounts of the T3S proteins ectopically expressed from plasmids. Examination of the corresponding mRNAs by real-time PCR revealed that the mRNAs readily decreased in the fasyn-deleted mutant and this suppressive effect on the mRNA levels appeared to spread across all lee operons. Complementation with fasyn reverted the T3S-deficient phenotype. Furthermore, this reversion was also seen when the mutant was supplemented with locus of enterocyte effacement activators (Ler or GrlA). Thus, these unique clustering genes located apart from locus of enterocyte effacement on the bacterial chromosome also play a role in affecting T3S of EHEC.

AB - Bioinformatics analysis was used to search for unknown genes that might influence the phenotypic presentations of enterohaemorrhagic Escherichia coli (EHEC). By so doing and using the known genomic data from EHEC O157: H7 and K-12, it has been deduced that genes Z4863 to Z4866 of EHEC do not exist in K-12 strains. These four gene sequences have low degrees of homology (18-40% amino acid identities) to a set of genes in K-12, which have been known to encode fatty acid biosynthesis enzymes. We referred these four consecutive genes as a fasyn cluster and found that deletion of fasyn from EHEC resulted in a defective type-III secretion (T3S). This deletion apparently did not decrease the amounts of the T3S proteins ectopically expressed from plasmids. Examination of the corresponding mRNAs by real-time PCR revealed that the mRNAs readily decreased in the fasyn-deleted mutant and this suppressive effect on the mRNA levels appeared to spread across all lee operons. Complementation with fasyn reverted the T3S-deficient phenotype. Furthermore, this reversion was also seen when the mutant was supplemented with locus of enterocyte effacement activators (Ler or GrlA). Thus, these unique clustering genes located apart from locus of enterocyte effacement on the bacterial chromosome also play a role in affecting T3S of EHEC.

UR - http://www.scopus.com/inward/record.url?scp=84992700520&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84992700520&partnerID=8YFLogxK

U2 - 10.1099/mic.0.000348

DO - 10.1099/mic.0.000348

M3 - Article

C2 - 27519956

AN - SCOPUS:84992700520

VL - 162

SP - 1744

EP - 1754

JO - Microbiology

JF - Microbiology

SN - 1350-0872

IS - 10

ER -