TY - JOUR
T1 - Untargeted foodomics strategy using high-resolution mass spectrometry reveals potential indicators for fish freshness
AU - Chang, William Chih Wei
AU - Wu, Hsin Yi
AU - Yeh, Yun
AU - Liao, Pao Chi
N1 - Funding Information:
This work was supported by the Ministry of Science and Technology, Taiwan [grant number MOST108-2113-M-006-008 and MOST109-2113-M-006-015 ]. The authors acknowledge the mass spectrometry analysis supported by the Metabolomics Core Facility, Scientific Instrument Center at Academia Sinica, NTU Consortia of Key Technologies and NTU Instrumentation center, and Instrument Center of National Cheng Kung University.
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/8/29
Y1 - 2020/8/29
N2 - Fish among other food can easily become spoilage. However, the existing literature endeavouring into a holistic investigation of fish metabolome during storage is scarce. There is a need for a powerful tool for more in-depth determinations. The present study is leveraging high-resolution mass spectrometry (HRMS)-based untargeted foodomics in the exploration of potential indicators for fish freshness. Three batches of fish fillets were stored in the refrigerator (+4 °C) for 0, 24, 48, and 72 h. Features were detected via UPLC-Q-Orbitrap HRMS and hereby undergone selection, identification, and verification. Eight sensitive indicators with significant time-dependent responses were yielded. The loss of freshness in fish is manifested in the upregulation of uracil, hypoxanthine, and inosine (involved in nucleotide changes) and α-linolenic acid, docosahexaenoic acid, arachidonic acid, and linoleic acid (involved in lipid hydrolysis) as well as in the downregulation of decanoylcarnitine (involved in fatty acid metabolism). Our work provides a promising approach to assess the quality of fish during storage and gain deeper insights into the metabolic reaction.
AB - Fish among other food can easily become spoilage. However, the existing literature endeavouring into a holistic investigation of fish metabolome during storage is scarce. There is a need for a powerful tool for more in-depth determinations. The present study is leveraging high-resolution mass spectrometry (HRMS)-based untargeted foodomics in the exploration of potential indicators for fish freshness. Three batches of fish fillets were stored in the refrigerator (+4 °C) for 0, 24, 48, and 72 h. Features were detected via UPLC-Q-Orbitrap HRMS and hereby undergone selection, identification, and verification. Eight sensitive indicators with significant time-dependent responses were yielded. The loss of freshness in fish is manifested in the upregulation of uracil, hypoxanthine, and inosine (involved in nucleotide changes) and α-linolenic acid, docosahexaenoic acid, arachidonic acid, and linoleic acid (involved in lipid hydrolysis) as well as in the downregulation of decanoylcarnitine (involved in fatty acid metabolism). Our work provides a promising approach to assess the quality of fish during storage and gain deeper insights into the metabolic reaction.
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U2 - 10.1016/j.aca.2020.06.016
DO - 10.1016/j.aca.2020.06.016
M3 - Article
C2 - 32800143
AN - SCOPUS:85087644126
SN - 0003-2670
VL - 1127
SP - 98
EP - 105
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
ER -