Urinalysis with molecularly imprinted poly(ethylene-co-vinyl alcohol) potentiostat sensors

Chun Yueh Huang, Tain Chin Tsai, James L. Thomas, Mei Hwa Lee, Bin-Da Liu, Hung Yin Lin

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Among many important biomarkers excreted in urine are albumin, uric acid, glucose, urea, creatine and creatinine. In the growing elderly population, these biomarkers may be useful correlates with kidney dysfunction, infection and related problems such as glomerular, proximal, and distal convoluted tubule functions, diabetes, hypertension and proteinuria. This study employed solvent evaporation processing of poly(ethylene-co-vinyl alcohol), (EVAL) to form molecularly imprinted polymers (MIPs) that recognize creatinine, urea, and lysozyme. The mole ratio of ethylene to vinyl alcohol affected the performance: 27 mol% ethylene gave the highest imprinting effectiveness for creatinine and urea, while 44 mol% gave the highest effectiveness for lysozyme. Electrochemical examination using a home made potentiostat and imprinted polymer electrode showed electrical signals responsive to the target molecules. Finally, an actual urine sample was tested using the electrode. The test results were compared with those of the commercial instrument ARCHITECT ci 8200 system to precisely determine the accuracy of the molecularly imprinted polymer electrode for urinalysis.

Original languageEnglish
Pages (from-to)2611-2617
Number of pages7
JournalBiosensors and Bioelectronics
Volume24
Issue number8
DOIs
Publication statusPublished - 2009 Apr 15

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Urinalysis
Urea
Creatinine
Polymers
Ethylene
Electrodes
Alcohols
Biomarkers
Muramidase
Sensors
Enzymes
Urine
Creatine
Medical problems
Uric Acid
Proteinuria
Glucose
Albumins
Evaporation
Hypertension

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Huang, C. Y., Tsai, T. C., Thomas, J. L., Lee, M. H., Liu, B-D., & Lin, H. Y. (2009). Urinalysis with molecularly imprinted poly(ethylene-co-vinyl alcohol) potentiostat sensors. Biosensors and Bioelectronics, 24(8), 2611-2617. https://doi.org/10.1016/j.bios.2009.01.016
Huang, Chun Yueh ; Tsai, Tain Chin ; Thomas, James L. ; Lee, Mei Hwa ; Liu, Bin-Da ; Lin, Hung Yin. / Urinalysis with molecularly imprinted poly(ethylene-co-vinyl alcohol) potentiostat sensors. In: Biosensors and Bioelectronics. 2009 ; Vol. 24, No. 8. pp. 2611-2617.
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Urinalysis with molecularly imprinted poly(ethylene-co-vinyl alcohol) potentiostat sensors. / Huang, Chun Yueh; Tsai, Tain Chin; Thomas, James L.; Lee, Mei Hwa; Liu, Bin-Da; Lin, Hung Yin.

In: Biosensors and Bioelectronics, Vol. 24, No. 8, 15.04.2009, p. 2611-2617.

Research output: Contribution to journalArticle

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