TY - JOUR
T1 - Urinary phthalate metabolites are associated with biomarkers of DNA damage and lipid peroxidation in pregnant women – Tainan Birth Cohort Study (TBCS)
AU - Waits, Alexander
AU - Chen, Hsin Chang
AU - Kuo, Pao Lin
AU - Wang, Chih Wen
AU - Huang, Han Bin
AU - Chang, Wei Hsiang
AU - Shih, Shu Fang
AU - Huang, Po Chin
N1 - Funding Information:
We thank all participating women for their generous collaboration. We thank to physicians, Dr. Chiung-Hsin Chang (M.D.), Dr. Chen-Hsiang Yu (M.D.) and Dr. Pao-Lin Kuo (M.D), and Ms. Cui-Bi Liao, the senior nurse, at the National Cheng Kung University Hospital, for their assistance of subject recruitment and specimen collection. We thank Ms. Wan-Ting Chang in National Health Research Institutes for sample pretreatment and data collection, and Mr. Chien-Jen Wang for his assistance in instrument analysis. We thank the National Health Research Institutes for grants: EM-108-PP-12, EM-109-PP-11 and partial funding from the Ministry of Science and Technology (Grant No.: MOST 106-3114-B-400-001 and MOST 108-2314-B-400-039).
Funding Information:
We thank all participating women for their generous collaboration. We thank to physicians, Dr. Chiung-Hsin Chang (M.D.), Dr. Chen-Hsiang Yu (M.D.) and Dr. Pao-Lin Kuo (M.D), and Ms. Cui-Bi Liao, the senior nurse, at the National Cheng Kung University Hospital, for their assistance of subject recruitment and specimen collection. We thank Ms. Wan-Ting Chang in National Health Research Institutes for sample pretreatment and data collection, and Mr. Chien-Jen Wang for his assistance in instrument analysis. We thank the National Health Research Institutes for grants: EM-108-PP-12 , EM-109-PP-11 and partial funding from the Ministry of Science and Technology (Grant No.: MOST 106-3114-B-400-001 and MOST 108-2314-B-400-039 ).
PY - 2020/9
Y1 - 2020/9
N2 - Phthalate exposure and oxidative stress have been linked to adverse reproductive outcomes in experimental studies, whereas no clear line has been drawn for human, especially in pregnant women. This study explored relationships between urinary phthalate metabolites and biomarkers of lipid peroxidation and oxidative and nitrosative DNA damage. Measurements from 97 Taiwanese pregnant women were taken at three different times during second and third trimesters. Five oxidative/nitrosative stress biomarkers – 8-hydroxy-2′-deoxyguanosine (8-OHdG), 8-nitroguanine (8-NO2Gua), 4-hydroxy-2-nonenal-mercapturic acid (HNE-MA), 8-isoprostaglandin F2α (8-isoPF2α), and malondialdehyde (MDA), and 11 phthalate metabolites were measured in urine samples. Linear regressions in each visit and linear mixed-model regressions were fitted to estimate percent changes in oxidative/nitrosative stress biomarkers resulting from inter-tertile increase of phthalate metabolite level and the cumulative concentrations of di (2-ethylhexyl) phthalate (DEHP) and dibutyl phthalate. The highest urine concentrations of phthalate metabolites and the greatest number of significant positive associations between phthalate metabolites and oxidative/nitrosative stress biomarkers were observed in the third visit and in repeated measurements analysis, respectively. Of the biomarkers related to DNA damage, 8-OHdG (25.4% inter-tertile increase for mono-iso-butyl phthalate) was more sensitive to phthalate exposure than 8-NO2Gua. Among the biomarkers of lipid peroxidation, HNE-MA (61.2% inter-tertile increase for sum of DEHP metabolites) was more sensitive than 8-isoPF2α and MDA. Our findings support the hypothesis that pregnant phthalate exposure increases the oxidative stress biomarkers of DNA damage and lipid peroxidation. Future research may elucidate the mediating roles of oxidative/nitrosative stress biomarkers in the link between phthalate exposure and adverse reproductive outcomes.
AB - Phthalate exposure and oxidative stress have been linked to adverse reproductive outcomes in experimental studies, whereas no clear line has been drawn for human, especially in pregnant women. This study explored relationships between urinary phthalate metabolites and biomarkers of lipid peroxidation and oxidative and nitrosative DNA damage. Measurements from 97 Taiwanese pregnant women were taken at three different times during second and third trimesters. Five oxidative/nitrosative stress biomarkers – 8-hydroxy-2′-deoxyguanosine (8-OHdG), 8-nitroguanine (8-NO2Gua), 4-hydroxy-2-nonenal-mercapturic acid (HNE-MA), 8-isoprostaglandin F2α (8-isoPF2α), and malondialdehyde (MDA), and 11 phthalate metabolites were measured in urine samples. Linear regressions in each visit and linear mixed-model regressions were fitted to estimate percent changes in oxidative/nitrosative stress biomarkers resulting from inter-tertile increase of phthalate metabolite level and the cumulative concentrations of di (2-ethylhexyl) phthalate (DEHP) and dibutyl phthalate. The highest urine concentrations of phthalate metabolites and the greatest number of significant positive associations between phthalate metabolites and oxidative/nitrosative stress biomarkers were observed in the third visit and in repeated measurements analysis, respectively. Of the biomarkers related to DNA damage, 8-OHdG (25.4% inter-tertile increase for mono-iso-butyl phthalate) was more sensitive to phthalate exposure than 8-NO2Gua. Among the biomarkers of lipid peroxidation, HNE-MA (61.2% inter-tertile increase for sum of DEHP metabolites) was more sensitive than 8-isoPF2α and MDA. Our findings support the hypothesis that pregnant phthalate exposure increases the oxidative stress biomarkers of DNA damage and lipid peroxidation. Future research may elucidate the mediating roles of oxidative/nitrosative stress biomarkers in the link between phthalate exposure and adverse reproductive outcomes.
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U2 - 10.1016/j.envres.2020.109863
DO - 10.1016/j.envres.2020.109863
M3 - Article
C2 - 32846647
AN - SCOPUS:85087724466
SN - 0013-9351
VL - 188
JO - Environmental Research
JF - Environmental Research
M1 - 109863
ER -