Use of a gradient-generating microfluidic device to rapidly determine a suitable glucose concentration for cell viability test

Chia Hsien Yeh, Chien Hsien Chen, Yu-Cheng Lin

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We successfully determined a suitable glucose concentration for endothelial cells (ECs) using a gradient-generating microfluidic chip and a micro-stamper that were fabricated using micro-electro-mechanical systems (MEMS) technology. Our strategy was to generate a stable concentration gradient in the observation area based on a microfluidic network and micro-mixers, which produced a concentration gradient under various flow rates. The areas for cell adhesion were delineated on a glass slide with a micro-stamper using the micro-contact printing (μCP) method. We also discuss which glucose concentration gradients are suitable for cell viability test (i.e., 0-0.2%, 0.05-0.15%, and 0.06-0.17%). After examining various concentration gradients, the suitable glucose concentration for EC's viability test was determined to range from 0.077% (4.2 mM) to 0.147% (8.16 mM). Higher or lower concentrations caused the ECs to atrophy or die. In this study, we describe a gradient-generating microfluidic chip that can be used to produce various drug concentrations for multi-concentration tests.

Original languageEnglish
Pages (from-to)1011-1018
Number of pages8
JournalMicrofluidics and Nanofluidics
Volume10
Issue number5
DOIs
Publication statusPublished - 2011 May 1

Fingerprint

microfluidic devices
viability
Microfluidics
glucose
Glucose
Endothelial cells
Cells
gradients
Cell adhesion
Printing
Flow rate
Glass
chips
Pharmaceutical Preparations
atrophy
chutes
printing
low concentrations
drugs
adhesion

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Materials Chemistry

Cite this

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Use of a gradient-generating microfluidic device to rapidly determine a suitable glucose concentration for cell viability test. / Yeh, Chia Hsien; Chen, Chien Hsien; Lin, Yu-Cheng.

In: Microfluidics and Nanofluidics, Vol. 10, No. 5, 01.05.2011, p. 1011-1018.

Research output: Contribution to journalArticle

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