Using Biomass of Starch-rich Transgenic Arabidopsis Vacuolar as Feedstock for Fermentative Hydrogen Production

Yung-Chong Lou, Chieh Lun Cheng, Chun Yen Chen, Li Fen Huang, Jo Shu Chang

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Cellulose is the major constitute of plant biomass and highly available in agricultural wastes and industrial effluents, thereby being a cost-effective feedstock for bioenergy production. However, most hydrogen producing bacteria (HPB) could not directly convert cellulosic materials (such as rice husk and rice straw) into hydrogen whereas most HPB could utilize sugar and starch for hydrogen production. In this work, we used an indigenous bacterial isolate Clostridium butyricum CGS2 as HPB, which could directly convert soluble starch into H2 with a maximum H2 production rate and a H2 yield of 205.07 ml H2/h/l and 6.46 mmol H2/g starch, respectively. However, C. butyricum CGS2 could not ferment pure cellulosic materials such as carboxymethyl cellulose and xylan. Moreover, we found that C. butyricum CGS2 could utilize rich husk to produce H2 at a rate of 13.19 ml H2/h/l due to the starch content in rice husk (H2 yield = 1.49 mmol H2/g rice husk). In contrast, since lacking starch content, rice straw cannot be converted to H2 by C. butyricum CGS2. The foregoing results suggest that increasing the starch content in the natural agricultural wastes may make them better feedstock for fermentative H2 production. Hence, a genetically modified plant (Arabidopsis vacuolar) was constructed to enhance its starch concentration. The starch concentration of mutant plant S1 increased to 10.67 mg/fresh weight, which is four times higher than that of wild type plant. Using mutant plant S1 as carbon source, C. butyricum CGS2 was able to give a high cumulative H2 production and H2 production rate of 285.4 ml H2/l and 43.6 ml/h/l, respectively. The cumulative H2 production and H2 production rate both increased when the concentration of the transgenic plant was increased. Therefore, this study successful demonstrated the feasibility of expressing starch on genetically-modified plants to create a more effective feedstock for dark H2 fermentation.

Original languageEnglish
Title of host publicationHydrogen Production Technologies - Part 1
EditorsDetlef Stolten, Thomas Grube
PublisherForschungszentrum Julich GmbH
Number of pages5
ISBN (Electronic)9783893366521
Publication statusPublished - 2010
Event18th World Hydrogen Energy Conference, WHEC 2010 - Essen, Germany
Duration: 2010 May 162010 May 21

Publication series

Name18th World Hydrogen Energy Conference 2010, WHEC 2010, Proceedings


Conference18th World Hydrogen Energy Conference, WHEC 2010

All Science Journal Classification (ASJC) codes

  • Energy Engineering and Power Technology
  • Fuel Technology
  • Renewable Energy, Sustainability and the Environment


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