TY - JOUR
T1 - Using impedance-sensing system to evaluate the effect of wound healing behavior on cells treated by vitamin C
AU - Lee, Hsiao Chen
AU - Lin, Chi Chang
AU - Yu, Shun Sheng
AU - Chang, Bin Wha
AU - Chang, Hsien-Chang
PY - 2009/12/1
Y1 - 2009/12/1
N2 - Burn injuries could be local or systemic problems, so multiple disciplines should be considered, including nutrition. Vitamin C is a cofactor of prolyl and lysyl hydroxylation for collagen maturation and ceramide synthesis and an important water-soluble free radical scavenger. Clinically, supplement of vitamin C is part of burn care for improving the healing process and decreasing further damage. With modified interdigitated microelectrode plate (IMP), electrical burn of MDCK cells was produced with constant current for few seconds. Healing process of cells damaged by different duration of direct constant current and damaged cells treated with 100 μM vitamin C were recorded by modified IMP and impedance-sensing system. The rate of change of conductivity is fast in the group of shorter exposure time and vitamin C-treated cells. Compared with the result of MTT test, we concluded that vitamin C decreased the duration of healing through increase cell proliferation.
AB - Burn injuries could be local or systemic problems, so multiple disciplines should be considered, including nutrition. Vitamin C is a cofactor of prolyl and lysyl hydroxylation for collagen maturation and ceramide synthesis and an important water-soluble free radical scavenger. Clinically, supplement of vitamin C is part of burn care for improving the healing process and decreasing further damage. With modified interdigitated microelectrode plate (IMP), electrical burn of MDCK cells was produced with constant current for few seconds. Healing process of cells damaged by different duration of direct constant current and damaged cells treated with 100 μM vitamin C were recorded by modified IMP and impedance-sensing system. The rate of change of conductivity is fast in the group of shorter exposure time and vitamin C-treated cells. Compared with the result of MTT test, we concluded that vitamin C decreased the duration of healing through increase cell proliferation.
UR - http://www.scopus.com/inward/record.url?scp=74349124090&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=74349124090&partnerID=8YFLogxK
U2 - 10.4015/S1016237209001611
DO - 10.4015/S1016237209001611
M3 - Article
AN - SCOPUS:74349124090
VL - 21
SP - 445
EP - 448
JO - Biomedical Engineering - Applications, Basis and Communications
JF - Biomedical Engineering - Applications, Basis and Communications
SN - 1016-2372
IS - 6
ER -