Using impedance-sensing system to evaluate the effect of wound healing behavior on cells treated by vitamin C

Burn injuries could be local or systemic problems, so multiple disciplines should be considered, including nutrition. Vitamin C is a cofactor of prolyl and lysyl hydroxylation for collagen maturation and ceramide synthesis and an important water-soluble free radical scavenger. Clinically, supplement of vitamin C is part of burn care for improving the healing process and decreasing further damage. With modified interdigitated microelectrode plate (IMP), electrical burn of MDCK cells was produced with constant current for few seconds. Healing process of cells damaged by different duration of direct constant current and damaged cells treated with 100 μM vitamin C were recorded by modified IMP and impedance-sensing system. The rate of change of conductivity is fast in the group of shorter exposure time and vitamin C-treated cells. Compared with the result of MTT test, we concluded that vitamin C decreased the duration of healing through increase cell proliferation.