Using magnetic nanoparticles to enhance gene transfection on magneto-electroporation microchips

Min Li, Yu Cheng Lin, Kai Chun Su

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

This study demonstrated that DNA associated with magnetic nanoparticles can be attracted to specific areas of cell surfaces under magnetic fields, which highly increased the DNA concentration at specific areas and further enhanced the gene transfection in an electroporation (EP) method. The superparamagnetic nanoparticle's distribution could be operated by magnetic field, where the gravity effect could be neglected. Compared with the electroporation with and without electrostatic attracting force, the magneto-electroporation with magnetic attracting force showed higher delivery rate (63.05 %) in the electroporation processes. Simulating an asymmetric magnetic field helps to create experiment environment with different intensities of magnetic flux density. The resultant difference can be identified by the profile of fluorescence. This report focused on enhancement and targeting of gene transfection using 6 nm γ-Fe2O3 nanoparticles and electroporation microchips.

Original languageEnglish
Title of host publicationProgress on Advanced Manufacture for Micro/Nano Technology 2005 - Proceedings of the 2005 International Conference on Advanced Manufacture
PublisherTrans Tech Publications Ltd
Pages661-666
Number of pages6
EditionPART 1
ISBN (Print)0878499903, 9780878499908
DOIs
Publication statusPublished - 2006
Event2005 International Conference on Advanced Manufacture, ICAM2005 - Taipei, R.O.C., Taiwan
Duration: 2005 Nov 282005 Dec 2

Publication series

NameMaterials Science Forum
NumberPART 1
Volume505-507
ISSN (Print)0255-5476
ISSN (Electronic)1662-9752

Other

Other2005 International Conference on Advanced Manufacture, ICAM2005
CountryTaiwan
CityTaipei, R.O.C.
Period05-11-2805-12-02

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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