Using magnetic nanoparticles to enhance site-specific gene transfection on magneto-electroporation microchips

Ming Kai Liu; Hung Yi Chen; Yi Lung Wang; Shen Shing Tsai; Min Li; Yu Cheng Lin

doi:10.1109/IECON.2007.4459934

Using magnetic nanoparticles to enhance site-specific gene transfection on magneto-electroporation microchips

Ming Kai Liu, Hung Yi Chen, Yi Lung Wang, Shen Shing Tsai, Min Li, Yu Cheng Lin

Department of Engineering Science

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

This study demonstrated that DNA associated with magnetic nanoparticles can be attracted to specific areas of cell surfaces under magnetic fields, which highly increased the DNA concentration at specific areas and further enhanced the gene transfection in an electroporation (EP) method. The superparamagnetic nanoparticle's distribution could be operated by magnetic field, where the gravity effect could be neglected. Compared with the electroporation with and without electrostatic attracting force, the magneto-electroporation with magnetic attracting force showed higher delivery rate (63.05%) in the electroporation processes. Simulating an asymmetric magnetic field helps to create experiment environment with different intensities of magnetic flux density. The resultant difference can be identified by the profile of fluorescence. This report focused on enhancement and targeting of gene transfection using 6 nm γ-Fe₂O₃ nanoparticles and electroporation microchips.

Original language	English
Title of host publication	Proceedings of the 33rd Annual Conference of the IEEE Industrial Electronics Society, IECON
Pages	3023-3026
Number of pages	4
DOIs	https://doi.org/10.1109/IECON.2007.4459934
Publication status	Published - 2007
Event	33rd Annual Conference of the IEEE Industrial Electronics Society, IECON - Taipei, Taiwan Duration: 2007 Nov 5 → 2007 Nov 8

Publication series

Name	IECON Proceedings (Industrial Electronics Conference)

Other

Other	33rd Annual Conference of the IEEE Industrial Electronics Society, IECON
Country/Territory	Taiwan
City	Taipei
Period	07-11-05 → 07-11-08

All Science Journal Classification (ASJC) codes

Control and Systems Engineering
Electrical and Electronic Engineering

Access to Document

10.1109/IECON.2007.4459934

Cite this

Liu, M. K., Chen, H. Y., Wang, Y. L., Tsai, S. S., Li, M., & Lin, Y. C. (2007). Using magnetic nanoparticles to enhance site-specific gene transfection on magneto-electroporation microchips. In Proceedings of the 33rd Annual Conference of the IEEE Industrial Electronics Society, IECON (pp. 3023-3026). Article 4459934 (IECON Proceedings (Industrial Electronics Conference)). https://doi.org/10.1109/IECON.2007.4459934

@inproceedings{c93e286cc3a54f1e85c34324d2d495c7,

title = "Using magnetic nanoparticles to enhance site-specific gene transfection on magneto-electroporation microchips",

abstract = "This study demonstrated that DNA associated with magnetic nanoparticles can be attracted to specific areas of cell surfaces under magnetic fields, which highly increased the DNA concentration at specific areas and further enhanced the gene transfection in an electroporation (EP) method. The superparamagnetic nanoparticle's distribution could be operated by magnetic field, where the gravity effect could be neglected. Compared with the electroporation with and without electrostatic attracting force, the magneto-electroporation with magnetic attracting force showed higher delivery rate (63.05%) in the electroporation processes. Simulating an asymmetric magnetic field helps to create experiment environment with different intensities of magnetic flux density. The resultant difference can be identified by the profile of fluorescence. This report focused on enhancement and targeting of gene transfection using 6 nm γ-Fe2O3 nanoparticles and electroporation microchips.",

author = "Liu, {Ming Kai} and Chen, {Hung Yi} and Wang, {Yi Lung} and Tsai, {Shen Shing} and Min Li and Lin, {Yu Cheng}",

year = "2007",

doi = "10.1109/IECON.2007.4459934",

language = "English",

isbn = "1424407834",

series = "IECON Proceedings (Industrial Electronics Conference)",

pages = "3023--3026",

booktitle = "Proceedings of the 33rd Annual Conference of the IEEE Industrial Electronics Society, IECON",

note = "33rd Annual Conference of the IEEE Industrial Electronics Society, IECON ; Conference date: 05-11-2007 Through 08-11-2007",

}

Liu, MK, Chen, HY, Wang, YL, Tsai, SS, Li, M & Lin, YC 2007, Using magnetic nanoparticles to enhance site-specific gene transfection on magneto-electroporation microchips. in Proceedings of the 33rd Annual Conference of the IEEE Industrial Electronics Society, IECON., 4459934, IECON Proceedings (Industrial Electronics Conference), pp. 3023-3026, 33rd Annual Conference of the IEEE Industrial Electronics Society, IECON, Taipei, Taiwan, 07-11-05. https://doi.org/10.1109/IECON.2007.4459934

Using magnetic nanoparticles to enhance site-specific gene transfection on magneto-electroporation microchips. / Liu, Ming Kai; Chen, Hung Yi; Wang, Yi Lung et al.
Proceedings of the 33rd Annual Conference of the IEEE Industrial Electronics Society, IECON. 2007. p. 3023-3026 4459934 (IECON Proceedings (Industrial Electronics Conference)).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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T1 - Using magnetic nanoparticles to enhance site-specific gene transfection on magneto-electroporation microchips

AU - Liu, Ming Kai

AU - Chen, Hung Yi

AU - Wang, Yi Lung

AU - Tsai, Shen Shing

AU - Li, Min

AU - Lin, Yu Cheng

PY - 2007

Y1 - 2007

N2 - This study demonstrated that DNA associated with magnetic nanoparticles can be attracted to specific areas of cell surfaces under magnetic fields, which highly increased the DNA concentration at specific areas and further enhanced the gene transfection in an electroporation (EP) method. The superparamagnetic nanoparticle's distribution could be operated by magnetic field, where the gravity effect could be neglected. Compared with the electroporation with and without electrostatic attracting force, the magneto-electroporation with magnetic attracting force showed higher delivery rate (63.05%) in the electroporation processes. Simulating an asymmetric magnetic field helps to create experiment environment with different intensities of magnetic flux density. The resultant difference can be identified by the profile of fluorescence. This report focused on enhancement and targeting of gene transfection using 6 nm γ-Fe2O3 nanoparticles and electroporation microchips.

AB - This study demonstrated that DNA associated with magnetic nanoparticles can be attracted to specific areas of cell surfaces under magnetic fields, which highly increased the DNA concentration at specific areas and further enhanced the gene transfection in an electroporation (EP) method. The superparamagnetic nanoparticle's distribution could be operated by magnetic field, where the gravity effect could be neglected. Compared with the electroporation with and without electrostatic attracting force, the magneto-electroporation with magnetic attracting force showed higher delivery rate (63.05%) in the electroporation processes. Simulating an asymmetric magnetic field helps to create experiment environment with different intensities of magnetic flux density. The resultant difference can be identified by the profile of fluorescence. This report focused on enhancement and targeting of gene transfection using 6 nm γ-Fe2O3 nanoparticles and electroporation microchips.

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Liu MK, Chen HY, Wang YL, Tsai SS, Li M, Lin YC. Using magnetic nanoparticles to enhance site-specific gene transfection on magneto-electroporation microchips. In Proceedings of the 33rd Annual Conference of the IEEE Industrial Electronics Society, IECON. 2007. p. 3023-3026. 4459934. (IECON Proceedings (Industrial Electronics Conference)). doi: 10.1109/IECON.2007.4459934

Using magnetic nanoparticles to enhance site-specific gene transfection on magneto-electroporation microchips

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