Using recombinant cyanobacterium (Synechococcus elongatus) with increased carbohydrate productivity as feedstock for bioethanol production via separate hydrolysis and fermentation process

Te Jin Chow, Hsiang Yen Su, Tsung Yu Tsai, Hsiang Hui Chou, Tse Min Lee, Jo Shu Chang

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

In this work, a recombinant cyanobacterium strain with increased photosynthesis rate, cell growth and carbohydrate production efficiency was genetically engineered by co-expressing ictB, ecaA, and acsAB (encoded for bacterial cellulose) in Synechococcus elongatus PCC7942. The resulting cyanobacterial biomass could be effectively hydrolyzed with dilute acid (2% sulfuric acid), achieving a nearly 90% glucose recovery at a biomass concentration of 80. g/L. Bioethanol can be produced from fermenting the acidic hydrolysate of S. elongatus PCC7942 via separate hydrolysis and fermentation (SHF) process at a concentration of 7.2. g/L and with a 91% theoretical yield.

Original languageEnglish
Pages (from-to)33-41
Number of pages9
JournalBioresource technology
Volume184
DOIs
Publication statusPublished - 2015 May 1

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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