Using surfaces to modulate the morphology and structure of attached cells-a case of cancer cells on chitosan membranes

Hung Hsun Shuai, Chung Yao Yang, I-Chen Hans, Roger L. York, Tzu Chun Liao, Wen Shiang Chen, J. Andrew Yeh, Chao Min Cheng

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

This paper describes the development of physically and/or chemically modified chitosan membranes to probe cellular behaviors and molecular-level structural responses of NIH-3T3 fibroblasts (normal cells) and Ha-ras-transformed cells (abnormal cells) adhered onto these modified membranes. To prepare chitosan membranes with nanometrically scaled physical features, we have demonstrated an inexpensive and easy-to-handle method that could be easily integrated with IC-based manufacturing processes with mass production potential. These physically or chemically modified chitosan membranes were examined via scanning electron microscopy, atomic force microscopy, X-ray photoelectron spectroscopy, and water contact angle measurement, in order to gain a better understanding of chitosan membrane surface characteristics including surface morphology, stiffness, functional groups, and surface hydrophobicity/ hydrophilicity. NIH-3T3 fibroblasts and their Ha-ras-transformed progeny were cultured on these modified chitosan membranes. After 12, 24 and 48 h of culture, these cells were investigated to decipher cellular behaviors. We found that NIH-3T3 fibroblasts and their Ha-ras-transformed progeny exhibited distinct structurally based responses attributable to chitosan membrane surface chemical or physical properties that we demonstrate as possibly applicable, for drug screening applications. Secondarily, but crucially to this study, we developed a chitosan-based micropatterning procedure that allowed us to re-arrange mammalian cells (i.e., HeLa cells in this study, for cancer drug screening) at the desired locations (with a single-cell array format). This procedure was based on cell affinity to different surface topographies of chitosan membranes that we prepared. This cell-based patterning approach has the potential for use in a wide range of applications including use as a promising platform for drug discovery, cytotoxicity studies, functional genomics, and investigations of cellular microenvironment. We believe that this study would provide further understanding of naturally derived biomaterials, lay the foundation for broadening the applications of chitosan, and facilitate the development of new biomedical devices (i.e., artificial stents, implantable artificial tissues, and sustainable implantable biosensors) with unique cell-material interface properties and characteristics, such as in vitro cell culture and diagnostic platforms.

Original languageEnglish
Pages (from-to)3058-3067
Number of pages10
JournalChemical Science
Volume4
Issue number8
DOIs
Publication statusPublished - 2013 Aug 1

Fingerprint

Chitosan
Cells
Membranes
Fibroblasts
Cell culture
Screening
Stents
Hydrophilicity
Biocompatible Materials
Surface topography
Cytotoxicity
Hydrophobicity
Angle measurement
Biosensors
Pharmaceutical Preparations
Chemical properties
Functional groups
Contact angle
Surface morphology
Atomic force microscopy

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

Shuai, Hung Hsun ; Yang, Chung Yao ; Hans, I-Chen ; York, Roger L. ; Liao, Tzu Chun ; Chen, Wen Shiang ; Yeh, J. Andrew ; Cheng, Chao Min. / Using surfaces to modulate the morphology and structure of attached cells-a case of cancer cells on chitosan membranes. In: Chemical Science. 2013 ; Vol. 4, No. 8. pp. 3058-3067.
@article{51c90e20b29b4ec8ba3a29ff648d9eaa,
title = "Using surfaces to modulate the morphology and structure of attached cells-a case of cancer cells on chitosan membranes",
abstract = "This paper describes the development of physically and/or chemically modified chitosan membranes to probe cellular behaviors and molecular-level structural responses of NIH-3T3 fibroblasts (normal cells) and Ha-ras-transformed cells (abnormal cells) adhered onto these modified membranes. To prepare chitosan membranes with nanometrically scaled physical features, we have demonstrated an inexpensive and easy-to-handle method that could be easily integrated with IC-based manufacturing processes with mass production potential. These physically or chemically modified chitosan membranes were examined via scanning electron microscopy, atomic force microscopy, X-ray photoelectron spectroscopy, and water contact angle measurement, in order to gain a better understanding of chitosan membrane surface characteristics including surface morphology, stiffness, functional groups, and surface hydrophobicity/ hydrophilicity. NIH-3T3 fibroblasts and their Ha-ras-transformed progeny were cultured on these modified chitosan membranes. After 12, 24 and 48 h of culture, these cells were investigated to decipher cellular behaviors. We found that NIH-3T3 fibroblasts and their Ha-ras-transformed progeny exhibited distinct structurally based responses attributable to chitosan membrane surface chemical or physical properties that we demonstrate as possibly applicable, for drug screening applications. Secondarily, but crucially to this study, we developed a chitosan-based micropatterning procedure that allowed us to re-arrange mammalian cells (i.e., HeLa cells in this study, for cancer drug screening) at the desired locations (with a single-cell array format). This procedure was based on cell affinity to different surface topographies of chitosan membranes that we prepared. This cell-based patterning approach has the potential for use in a wide range of applications including use as a promising platform for drug discovery, cytotoxicity studies, functional genomics, and investigations of cellular microenvironment. We believe that this study would provide further understanding of naturally derived biomaterials, lay the foundation for broadening the applications of chitosan, and facilitate the development of new biomedical devices (i.e., artificial stents, implantable artificial tissues, and sustainable implantable biosensors) with unique cell-material interface properties and characteristics, such as in vitro cell culture and diagnostic platforms.",
author = "Shuai, {Hung Hsun} and Yang, {Chung Yao} and I-Chen Hans and York, {Roger L.} and Liao, {Tzu Chun} and Chen, {Wen Shiang} and Yeh, {J. Andrew} and Cheng, {Chao Min}",
year = "2013",
month = "8",
day = "1",
doi = "10.1039/c3sc50533b",
language = "English",
volume = "4",
pages = "3058--3067",
journal = "Chemical Science",
issn = "2041-6520",
publisher = "Royal Society of Chemistry",
number = "8",

}

Using surfaces to modulate the morphology and structure of attached cells-a case of cancer cells on chitosan membranes. / Shuai, Hung Hsun; Yang, Chung Yao; Hans, I-Chen; York, Roger L.; Liao, Tzu Chun; Chen, Wen Shiang; Yeh, J. Andrew; Cheng, Chao Min.

In: Chemical Science, Vol. 4, No. 8, 01.08.2013, p. 3058-3067.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Using surfaces to modulate the morphology and structure of attached cells-a case of cancer cells on chitosan membranes

AU - Shuai, Hung Hsun

AU - Yang, Chung Yao

AU - Hans, I-Chen

AU - York, Roger L.

AU - Liao, Tzu Chun

AU - Chen, Wen Shiang

AU - Yeh, J. Andrew

AU - Cheng, Chao Min

PY - 2013/8/1

Y1 - 2013/8/1

N2 - This paper describes the development of physically and/or chemically modified chitosan membranes to probe cellular behaviors and molecular-level structural responses of NIH-3T3 fibroblasts (normal cells) and Ha-ras-transformed cells (abnormal cells) adhered onto these modified membranes. To prepare chitosan membranes with nanometrically scaled physical features, we have demonstrated an inexpensive and easy-to-handle method that could be easily integrated with IC-based manufacturing processes with mass production potential. These physically or chemically modified chitosan membranes were examined via scanning electron microscopy, atomic force microscopy, X-ray photoelectron spectroscopy, and water contact angle measurement, in order to gain a better understanding of chitosan membrane surface characteristics including surface morphology, stiffness, functional groups, and surface hydrophobicity/ hydrophilicity. NIH-3T3 fibroblasts and their Ha-ras-transformed progeny were cultured on these modified chitosan membranes. After 12, 24 and 48 h of culture, these cells were investigated to decipher cellular behaviors. We found that NIH-3T3 fibroblasts and their Ha-ras-transformed progeny exhibited distinct structurally based responses attributable to chitosan membrane surface chemical or physical properties that we demonstrate as possibly applicable, for drug screening applications. Secondarily, but crucially to this study, we developed a chitosan-based micropatterning procedure that allowed us to re-arrange mammalian cells (i.e., HeLa cells in this study, for cancer drug screening) at the desired locations (with a single-cell array format). This procedure was based on cell affinity to different surface topographies of chitosan membranes that we prepared. This cell-based patterning approach has the potential for use in a wide range of applications including use as a promising platform for drug discovery, cytotoxicity studies, functional genomics, and investigations of cellular microenvironment. We believe that this study would provide further understanding of naturally derived biomaterials, lay the foundation for broadening the applications of chitosan, and facilitate the development of new biomedical devices (i.e., artificial stents, implantable artificial tissues, and sustainable implantable biosensors) with unique cell-material interface properties and characteristics, such as in vitro cell culture and diagnostic platforms.

AB - This paper describes the development of physically and/or chemically modified chitosan membranes to probe cellular behaviors and molecular-level structural responses of NIH-3T3 fibroblasts (normal cells) and Ha-ras-transformed cells (abnormal cells) adhered onto these modified membranes. To prepare chitosan membranes with nanometrically scaled physical features, we have demonstrated an inexpensive and easy-to-handle method that could be easily integrated with IC-based manufacturing processes with mass production potential. These physically or chemically modified chitosan membranes were examined via scanning electron microscopy, atomic force microscopy, X-ray photoelectron spectroscopy, and water contact angle measurement, in order to gain a better understanding of chitosan membrane surface characteristics including surface morphology, stiffness, functional groups, and surface hydrophobicity/ hydrophilicity. NIH-3T3 fibroblasts and their Ha-ras-transformed progeny were cultured on these modified chitosan membranes. After 12, 24 and 48 h of culture, these cells were investigated to decipher cellular behaviors. We found that NIH-3T3 fibroblasts and their Ha-ras-transformed progeny exhibited distinct structurally based responses attributable to chitosan membrane surface chemical or physical properties that we demonstrate as possibly applicable, for drug screening applications. Secondarily, but crucially to this study, we developed a chitosan-based micropatterning procedure that allowed us to re-arrange mammalian cells (i.e., HeLa cells in this study, for cancer drug screening) at the desired locations (with a single-cell array format). This procedure was based on cell affinity to different surface topographies of chitosan membranes that we prepared. This cell-based patterning approach has the potential for use in a wide range of applications including use as a promising platform for drug discovery, cytotoxicity studies, functional genomics, and investigations of cellular microenvironment. We believe that this study would provide further understanding of naturally derived biomaterials, lay the foundation for broadening the applications of chitosan, and facilitate the development of new biomedical devices (i.e., artificial stents, implantable artificial tissues, and sustainable implantable biosensors) with unique cell-material interface properties and characteristics, such as in vitro cell culture and diagnostic platforms.

UR - http://www.scopus.com/inward/record.url?scp=84880020407&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84880020407&partnerID=8YFLogxK

U2 - 10.1039/c3sc50533b

DO - 10.1039/c3sc50533b

M3 - Article

AN - SCOPUS:84880020407

VL - 4

SP - 3058

EP - 3067

JO - Chemical Science

JF - Chemical Science

SN - 2041-6520

IS - 8

ER -