Using the biosensor based on impedance measurement and sandwich immunoassay for carcinoembryonic antigen detection

Chia Hsien Yeh, Kuan Feng Su, Yu-Cheng Lin, Pi Lan Shen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study, a biosensor was successfully used to detect the impedance signals of various carcinoembryonic antigen (CEA) concentrations based on the developed sandwich-immunoassay method. CEA is a type of protein molecule that can be found in many different cells of the body, but is typically associated with certain tumors; therefore, CEA concentration detection is important for quantitative analysis. In this study, the gold nanoparticles (AuNPs) were introduced into a biosensor through the specific binding of antibodies, thus creating a bridge between two electrodes allowing electrons to move between them. When the concentration of the anti-CEA antibody-colloidal gold conjugates was diluted 30 times, the detectable limit for CEA concentration was 1 ng/mL with 1 μg/mL of anti-CEA antibody. Therefore, the developed biosensor is advantageous because it is effective with small sample volumes (25 μL), is a form of rapid quantitative measurement, and works with detection concentrations range in clinical diagnosis.

Original languageEnglish
Title of host publicationIEEE SENSORS 2013 - Proceedings
PublisherIEEE Computer Society
ISBN (Print)9781467346405
DOIs
Publication statusPublished - 2013 Jan 1
Event12th IEEE SENSORS 2013 Conference - Baltimore, MD, United States
Duration: 2013 Nov 42013 Nov 6

Publication series

NameProceedings of IEEE Sensors

Other

Other12th IEEE SENSORS 2013 Conference
CountryUnited States
CityBaltimore, MD
Period13-11-0413-11-06

Fingerprint

Antigens
Biosensors
Antibodies
Gold
Tumors
Nanoparticles
Proteins
Electrodes
Molecules
Electrons
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

Cite this

Yeh, C. H., Su, K. F., Lin, Y-C., & Shen, P. L. (2013). Using the biosensor based on impedance measurement and sandwich immunoassay for carcinoembryonic antigen detection. In IEEE SENSORS 2013 - Proceedings [6688210] (Proceedings of IEEE Sensors). IEEE Computer Society. https://doi.org/10.1109/ICSENS.2013.6688210
Yeh, Chia Hsien ; Su, Kuan Feng ; Lin, Yu-Cheng ; Shen, Pi Lan. / Using the biosensor based on impedance measurement and sandwich immunoassay for carcinoembryonic antigen detection. IEEE SENSORS 2013 - Proceedings. IEEE Computer Society, 2013. (Proceedings of IEEE Sensors).
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Yeh, CH, Su, KF, Lin, Y-C & Shen, PL 2013, Using the biosensor based on impedance measurement and sandwich immunoassay for carcinoembryonic antigen detection. in IEEE SENSORS 2013 - Proceedings., 6688210, Proceedings of IEEE Sensors, IEEE Computer Society, 12th IEEE SENSORS 2013 Conference, Baltimore, MD, United States, 13-11-04. https://doi.org/10.1109/ICSENS.2013.6688210

Using the biosensor based on impedance measurement and sandwich immunoassay for carcinoembryonic antigen detection. / Yeh, Chia Hsien; Su, Kuan Feng; Lin, Yu-Cheng; Shen, Pi Lan.

IEEE SENSORS 2013 - Proceedings. IEEE Computer Society, 2013. 6688210 (Proceedings of IEEE Sensors).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Yeh CH, Su KF, Lin Y-C, Shen PL. Using the biosensor based on impedance measurement and sandwich immunoassay for carcinoembryonic antigen detection. In IEEE SENSORS 2013 - Proceedings. IEEE Computer Society. 2013. 6688210. (Proceedings of IEEE Sensors). https://doi.org/10.1109/ICSENS.2013.6688210