Using the biosensor based on impedance measurement and sandwich immunoassay for carcinoembryonic antigen detection

Chia Hsien Yeh, Kuan Feng Su, Yu Cheng Lin, Pi Lan Shen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study, a biosensor was successfully used to detect the impedance signals of various carcinoembryonic antigen (CEA) concentrations based on the developed sandwich-immunoassay method. CEA is a type of protein molecule that can be found in many different cells of the body, but is typically associated with certain tumors; therefore, CEA concentration detection is important for quantitative analysis. In this study, the gold nanoparticles (AuNPs) were introduced into a biosensor through the specific binding of antibodies, thus creating a bridge between two electrodes allowing electrons to move between them. When the concentration of the anti-CEA antibody-colloidal gold conjugates was diluted 30 times, the detectable limit for CEA concentration was 1 ng/mL with 1 μg/mL of anti-CEA antibody. Therefore, the developed biosensor is advantageous because it is effective with small sample volumes (25 μL), is a form of rapid quantitative measurement, and works with detection concentrations range in clinical diagnosis.

Original languageEnglish
Title of host publicationIEEE SENSORS 2013 - Proceedings
PublisherIEEE Computer Society
ISBN (Print)9781467346405
DOIs
Publication statusPublished - 2013 Jan 1
Event12th IEEE SENSORS 2013 Conference - Baltimore, MD, United States
Duration: 2013 Nov 42013 Nov 6

Publication series

NameProceedings of IEEE Sensors

Other

Other12th IEEE SENSORS 2013 Conference
CountryUnited States
CityBaltimore, MD
Period13-11-0413-11-06

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

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