TY - GEN
T1 - Using the biosensor based on impedance measurement and sandwich immunoassay for carcinoembryonic antigen detection
AU - Yeh, Chia Hsien
AU - Su, Kuan Feng
AU - Lin, Yu Cheng
AU - Shen, Pi Lan
PY - 2013
Y1 - 2013
N2 - In this study, a biosensor was successfully used to detect the impedance signals of various carcinoembryonic antigen (CEA) concentrations based on the developed sandwich-immunoassay method. CEA is a type of protein molecule that can be found in many different cells of the body, but is typically associated with certain tumors; therefore, CEA concentration detection is important for quantitative analysis. In this study, the gold nanoparticles (AuNPs) were introduced into a biosensor through the specific binding of antibodies, thus creating a bridge between two electrodes allowing electrons to move between them. When the concentration of the anti-CEA antibody-colloidal gold conjugates was diluted 30 times, the detectable limit for CEA concentration was 1 ng/mL with 1 μg/mL of anti-CEA antibody. Therefore, the developed biosensor is advantageous because it is effective with small sample volumes (25 μL), is a form of rapid quantitative measurement, and works with detection concentrations range in clinical diagnosis.
AB - In this study, a biosensor was successfully used to detect the impedance signals of various carcinoembryonic antigen (CEA) concentrations based on the developed sandwich-immunoassay method. CEA is a type of protein molecule that can be found in many different cells of the body, but is typically associated with certain tumors; therefore, CEA concentration detection is important for quantitative analysis. In this study, the gold nanoparticles (AuNPs) were introduced into a biosensor through the specific binding of antibodies, thus creating a bridge between two electrodes allowing electrons to move between them. When the concentration of the anti-CEA antibody-colloidal gold conjugates was diluted 30 times, the detectable limit for CEA concentration was 1 ng/mL with 1 μg/mL of anti-CEA antibody. Therefore, the developed biosensor is advantageous because it is effective with small sample volumes (25 μL), is a form of rapid quantitative measurement, and works with detection concentrations range in clinical diagnosis.
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U2 - 10.1109/ICSENS.2013.6688210
DO - 10.1109/ICSENS.2013.6688210
M3 - Conference contribution
AN - SCOPUS:84893941872
SN - 9781467346405
T3 - Proceedings of IEEE Sensors
BT - IEEE SENSORS 2013 - Proceedings
PB - IEEE Computer Society
T2 - 12th IEEE SENSORS 2013 Conference
Y2 - 4 November 2013 through 6 November 2013
ER -