TY - JOUR
T1 - Using the electro-microchip for rapid quantitative analysis of the ketamine and luteinizing hormone detection
AU - Yeh, C. H.
AU - Sun, Z. H.
AU - Wang, W. T.
AU - Su, P. T.
AU - Lin, Y. C.
N1 - Funding Information:
The authors would like to thank the Center for Micro/Nano Technology, National Cheng Kung University, Tainan, Taiwan, R.O.C., for access to equipment and technical support. This research was partially supported by the Southern Taiwan Science Park Administration (STSPA), Taiwan, R.O.C. under contract no. EZ-10-09-44-98, and the National Science Council under grants 99RC06. It is also partially supported by Ministry of Economic Affairs, Taiwan, under grant 99-EC-17-A-19-S1-133.
PY - 2011
Y1 - 2011
N2 - This study successfully developed the electrode microchip to rapidly detect the ketamine (KET) and luteinizing hormone (LH) concentration by using the competitive and sandwich immunoassay, respectively. Our strategy was that the AuNPs were introduced into the electro-microchip by the specific binding of the antibodies and constructs a "bridge" between two electrodes allowing the electrons to pass. When the detection frequency was increased from 100 Hz to 1000 kHz, the clearly impedance distinguishment was occurred at 100 Hz. The detectable concentration limits for KET and LH were 50 ng/mL and 20 mIU/mL, respectively. Therefore, the developed electro-microchip has advantages for the smaller sample volume (30 μL), rapid quantitative measurement, and smaller detectable concentration than the commercial detection products (KET: 100 ng/mL, and LH: 80 mIU/mL).
AB - This study successfully developed the electrode microchip to rapidly detect the ketamine (KET) and luteinizing hormone (LH) concentration by using the competitive and sandwich immunoassay, respectively. Our strategy was that the AuNPs were introduced into the electro-microchip by the specific binding of the antibodies and constructs a "bridge" between two electrodes allowing the electrons to pass. When the detection frequency was increased from 100 Hz to 1000 kHz, the clearly impedance distinguishment was occurred at 100 Hz. The detectable concentration limits for KET and LH were 50 ng/mL and 20 mIU/mL, respectively. Therefore, the developed electro-microchip has advantages for the smaller sample volume (30 μL), rapid quantitative measurement, and smaller detectable concentration than the commercial detection products (KET: 100 ng/mL, and LH: 80 mIU/mL).
UR - http://www.scopus.com/inward/record.url?scp=84863118346&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84863118346&partnerID=8YFLogxK
U2 - 10.1016/j.proeng.2011.12.227
DO - 10.1016/j.proeng.2011.12.227
M3 - Conference article
AN - SCOPUS:84863118346
VL - 25
SP - 924
EP - 927
JO - Procedia Engineering
JF - Procedia Engineering
SN - 1877-7058
T2 - 25th Eurosensors Conference
Y2 - 4 September 2011 through 7 September 2011
ER -