This study successfully developed the electrode microchip to rapidly detect the ketamine (KET) and luteinizing hormone (LH) concentration by using the competitive and sandwich immunoassay, respectively. Our strategy was that the AuNPs were introduced into the electro-microchip by the specific binding of the antibodies and constructs a "bridge" between two electrodes allowing the electrons to pass. When the detection frequency was increased from 100 Hz to 1000 kHz, the clearly impedance distinguishment was occurred at 100 Hz. The detectable concentration limits for KET and LH were 50 ng/mL and 20 mIU/mL, respectively. Therefore, the developed electro-microchip has advantages for the smaller sample volume (30 μL), rapid quantitative measurement, and smaller detectable concentration than the commercial detection products (KET: 100 ng/mL, and LH: 80 mIU/mL).
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