We have successfully fabricated the micro-array electrode chip with different gaps by MEMS technology and generated the moving electric fields to drive cells migration by employing AC signals. Our strategy is to simulate the ramp waveform signal in the chip with various gaps (10 μm, 20 μm, and 30 μm) to find the optimal moving electric field by the ANSYS software. The electrode gap of 10 μm could generate the better moving electric field effect on cell migration than that of other gap sizes. In experiments, when fixing the electric field intensity at 0.6 V/mm in 100 kHz, the migration velocity of the cells was 21.25 μm/hr in 10 μm micro-assay electrode chip. Under the driving frequency influence, the cell migration was more obvious in excess of 50 kHz. This method for cell migration could be applied to cell manipulation of bio-applications.
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